Product number: NCM0017

MacConkey Agar is used for the isolation and differentiation of Gram-negative enteric bacilli in a laboratory setting. Conforms to Harmonized USP/EP/JP Requirements and FDA/BAM. MacConkey Agar is not intended for use in the diagnosis of disease or other conditions in humans.

A medium recommended by the Harmonized USP/EP/JP for isolation and identification of Escherichia coli from non-sterile products. Conforms to USP/EP/JP performance specification. Gelatin serves as source of carbon and nitrogen. Lactose is a fermentable carbohydrate and sodium chloride maintains the osmotic balance. Bile salts and crystal violet act as selective agents inhibiting many Gram-positive bacteria. Escherichia coli can ferment lactose to produce acid which results in a pH drop. This is indicated by neutral red resulting in pink colonies. Enough acid production will cause the precipitation of bile salts resulting in a bile precipitate or halo around lactose fermenting bacteria. Non-lactose fermenting bacteria such as Salmonella spp. grow but remain colorless with no bile precipitate. According to the Harmonized USP/EP/JP, MacConkey Broth is used as a selective enrichment broth, with subculture performed onto MacConkey Agar.


Formula Liter
Enzymatic Digest of Gelatin 17.0 g/L
Enzymatic Digest of Casein 1.5 g/L
Enzymatic Digest of Animal Tissue 1.5 g/L
Lactose 10.0 g/L
Bile Salts Mixture 1.5 g/L
Sodium Chloride 5.0 g/L
Neutral Red 0.03 g/L
Crystal Violet 0.001 g/L
Agar 13.5 g/L

Final pH: 7.1 ± 0.2 at 25ºC
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 50 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121ºC for 15 minutes.
  4. Cool to 45-50°C.

Product was previously known as Acumedia Product: 7102 MacConkey Agar; and LabM Product: HP006 MacConkey Agar (USP/EP/JP)

Order Details

Product # Product Description
NCM0017A MacConkey Agar, 500 g
NCM0017B MacConkey Agar, 5 kg
NCM0017C MacConkey Agar, 10 kg
NCM0017D MacConkey Agar, 25 kg (European only size)
NCM0017E MacConkey Agar, 50 kg (US only size)

Reference Material

NCM0017 - MacConkey Agar, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Hungarian] [Italian] [Spanish, Mexico] [Swedish]
NCM0017 - MacConkey Agar, Technical Specifications Sheet [English]

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