Product number: NCM0123

m-Endo Agar is used for the enumeration of coliforms in water by the membrane filtration method in a laboratory setting. m-Endo Agar is not intended for use in the diagnosis of disease or other conditions in humans.

The coliform group, especially Escherichia coli, are used as indicators of fecal pollution in water, for assessing the effectiveness of water treatment and disinfection, and for monitoring water quality. m-Endo Agar is prepared according to the formula of McCarthy, Delaney, and Grasso, and used in testing water for coliforms by a two-step membrane filtration procedure. Lauryl Tryptose Broth is used as the preliminary enrichment, resulting in higher coliform counts.

The American Public Health Association (APHA) recommends m-Endo Agar in standard total coliform membrane filtration procedure for testing water, wastewater, and foods. The US EPA specifies using m-Endo Agar in the total coliform methods for testing water. m-Endo Agar is also known as LES (Lawrence Experimental Station) Endo Agar.


Formula Liter
Lactose 9.4 g/L
Tryptose 7.5 g/L
Enzymatic Digest of Casein 3.7 g/L
Enzymatic Digest of Animal Tissue 3.7 g/L
Sodium Chloride 3.7 g/L
Potassium Phosphate, Dibasic 3.3 g/L
Sodium Sulfite 1.6 g/L
Yeast Extract 1.2 g/L
Potassium Phosphate, Monobasic 1.0 g/L
Basic Fuchsin 0.8 g/L
Sodium Deoxycholate 0.1 g/L
Sodium Lauryl Sulfate 0.05 g/L
Agar 15.0 g/L

Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 51 g of the medium in 1 liter of purified water containing 20 mL of non-denatured Ethanol.
  2. Heat with frequent agitation and boil to completely dissolve the medium.
  3. Avoid overheating. DO NOT AUTOCLAVE.

Product was previously known as Acumedia Product: 7724 m-Endo Agar; and LabM Product: LAB060 m-Endo Agar

Order Details

Product # Product Description
NCM0123A m-Endo Agar, 500 g
NCM0123B m-Endo Agar, 5 kg
NCM0123C m-Endo Agar, 10 kg
NCM0123D m-Endo Agar, 25 kg (European only size)
NCM0123E m-Endo Agar, 50 kg (US only size)

Reference Material

NCM0123 - m-Endo Agar, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0123 - m-Endo Agar, Technical Specifications Sheet [English]
7724 - m-Endo Agar, Product Information Sheet [English] [Portuguese]

For additional information or questions about product SDS documents, please contact Neogen at

Please check with your local representative for availability of these products within your region.