Product number: NCM0140

Lysine Iron Agar is used for the differentiation of microorganisms on the basis of lysine decarboxylase and hydrogen sulfide production. Lysine Iron Agar is not intended for use in the diagnosis of disease or other conditions in humans.

Lysine Iron Agar is prepared according to the formulation of Edwards and Fife, who developed the medium to detect Salmonella arizonae. S. arizonae ferments lactose rapidly, and the authors found expected H2S production on Triple Sugar Iron Agar was suppressed. Detection of S. arizonae is important because it has been implicated in food borne infections. By eliminating lactose and incorporating lysine, Edwards and Fife devised a medium differentiating enteric bacilli based on their ability to decarboxylate or deaminate lysine and produce abundant hydrogen sulfide. This medium is recommended for detecting rapid lactose-fermenting S. arizonae.


Formula Liter
Enzymatic Digest of Gelatin 5.0 g/L
Yeast Extract 3.0 g/L
Dextrose (Glucose) 1.0 g/L
L-Lysine 10.0 g/L
Ferric Ammonium Citrate 0.5 g/L
Sodium Thiosulfate 0.04 g/L
Bromocresol Purple 0.02 g/L
*Agar 13.5 g/L

Final pH: 6.7 ± 0.2 at 25°C
*10 -15 g according to gel strength
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 33 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Dispense into test tubes and autoclave at 121°C for 15 minutes.
  4. After autoclaving, allow medium to solidify in a slanted position.

Product was previously known as Acumedia Product: 7211 Lysine Iron Agar; and LabM Product: LAB054 Lysine Iron Agar

Order Details

Product # Product Description
NCM0140A Lysine Iron Agar, 500 g
NCM0140B Lysine Iron Agar, 5 kg
NCM0140C Lysine Iron Agar, 10 kg
NCM0140D Lysine Iron Agar, 25 kg (European only size)
NCM0140E Lysine Iron Agar, 50 kg (US only size)

Reference Material

NCM0140 - Lysine Iron Agar, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0140 - Lysine Iron Agar, Technical Specifications Sheet [English]

For additional information or questions about product SDS documents, please contact Neogen at

Please check with your local representative for availability of these products within your region.