Product number: NCM0116

Letheen Broth is primarily used for assessing the bactericidal activity of quaternary ammonium compounds, and is not intended for use in the diagnosis of disease or other conditions in humans.

Letheen Broth is used to determine the phenol co-efficient of cationic surfactants as recommended by the Official Methods of Analysis of the Association of Official Analytical Chemists (AOAC). It is also used in hygiene swabbing protocols where it is necessary to neutralize quaternary ammonium compounds.

A modification of FDA Broth, Letheen Broth contains lecithin to neutralize quaternary ammonium compounds and Polysorbate 80 to neutralize phenols, hexachlorophene, formalin and (with lecithin) ethanol.

Letheen Broth is easily prepared and has a clear appearance aiding in visual inspection for growth.

The American Society for Testing Materials (ASTM) specifies the use of Letheen Broth in the Standard Test Method for Preservatives in Water Containing Cosmetics.


Formula Liter
Peptone 10.0 g/L
Beef Extract 5.0 g/L
Sodium Chloride 5.0 g/L
Lecithin 0.7 g/L
Polysorbate 80 5.0 g/L

Final pH: 7.0 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Dissolve 25.7 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation to completely dissolve the medium if necessary.
  3. Dispense into final containers.
  4. Autoclave at 121°C for 15 minutes.

Product was previously known as LabM Product: LAB184 Letheen Broth

Order Details

Product # Product Description
NCM0116A Letheen Broth (with tween), 500 g
NCM0116B Letheen Broth (with tween), 5 kg
NCM0116C Letheen Broth (with tween), 10 kg
NCM0116D Letheen Broth (with tween), 25 kg (European only size)
NCM0116E Letheen Broth (with tween), 50 kg (US only size)

Reference Material

NCM0116 - Letheen Broth (with tween), Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Hungarian] [Italian] [Spanish, Mexico] [Swedish]
NCM0116 - Letheen Broth (with tween), Technical Specifications Sheet [English]

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