Product number: NCM0035
Lactobacilli MRS Agar is used for the cultivation of lactobacilli and is not intended for use in the diagnosis of disease or other conditions in humans.
MRS Agar is a medium for the cultivation and enumeration of Lactobacillus spp. Originally developed in 1960 by de Man, Rogosa & Sharpe, the medium is suitable for most lactic acid bacteria and is intended as a substitute for Tomato Juice Agar. When acidified to pH 5.4 MRS Agar can be used to enumerate Lactobacillus bulgaricus in yogurts.
Nutrition is provided by a mixture of carefully selected peptones, glucose, beef & yeast extracts while Tween® 80, magnesium and manganese sulfates act as growth stimulants. Selectivity against streptococci & molds is provided by ammonium citrate and sodium acetate. Used at low pH, ammonium citrate allows growth of lactobacilli while inhibiting a number of other microorganism groups. Occasionally, sterilization of this medium at 121°C for 15 minutes, in some autoclaves, may cause the pH to fall outside of the specified pH limits 6.4 +/- 0.2. In these rare cases, adjustment of the medium using acetic acid or sodium hydroxide is recommended.
|Yeast Extract||5.0 g/L|
|Beef Extract||10.0 g/L|
|Dipotassium Phosphate||2.0 g/L|
|Sodium Acetate||5.0 g/L|
|Triammonium Citrate||2.0 g/L|
|Magnesium Sulfate||0.2 g/L|
|Manganese Sulfate||0.05 g/L|
|Tween 80||1.08 g/L|
Final pH: 6.4 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
- Suspend 70 grams of the medium in one liter of purified water.
- Heat with frequent agitation to completely dissolve the medium, if necessary.
- Autoclave at 121°C for 15 minutes.
- Cool to 45-50°C.
- If acidified medium is required, adjust pH aseptically prior to pouring.
Product was previously known as Acumedia Product: 7543 Lactobacilli MRS Agar; and LabM Product: LAB093 M.R.S Agar - de Man, Rogosa and Sharpe Agar
|Product #||Product Description|
|NCM0035A||Lactobacilli MRS Agar, 500 g|
|NCM0035B||Lactobacilli MRS Agar, 5 kg|
|NCM0035C||Lactobacilli MRS Agar, 10 kg|
|NCM0035D||Lactobacilli MRS Agar, 25 kg (European only size)|
|NCM0035E||Lactobacilli MRS Agar, 50 kg (US only size)|
7543 - Lactobacilli MRS Agar, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0035 - Lactobacilli MRS Agar, Technical Specifications Sheet [English]
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