Product number: NCM0198

Kanamycin Aesculin Azide Agar is a selective isolation and enumeration medium for enterococci (Lancefield group D streptococci) in food, and is not intended for use in the diagnosis of disease or other conditions in humans.

Sodium azide and kanamycin provide the selective inhibition required whilst aesculin and iron salts form an indicator system for the presumptive identification of enterococci. Incubation at 42°C will increase the medium’s selectivity.


Formula Liter
Tryptone 20.0 g/L
Yeast Extract 5.0 g/L
Sodium Chloride 5.0 g/L
Sodium Citrate 1.0 g/L
Aesculin 1.0 g/L
Ferric Ammonium Citrate 0.5 g/L
Sodium Azide 0.15 g/L
Kanamycin Sulphate 0.02 g/L
Agar 10.0 g/L

Final pH: 7.0 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 43 grams of the medium in one 1 liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C.

Product was previously known as LabM Product: LAB106 Kanamycin Aesculin Azide Agar

Order Details

Product # Product Description
NCM0198A Kanamycin Aesculin Azide Agar, 500 g
NCM0198B Kanamycin Aesculin Azide Agar, 5 kg
NCM0198C Kanamycin Aesculin Azide Agar, 10 kg
NCM0198D Kanamycin Aesculin Azide Agar, 25 kg (European only size)
NCM0198E Kanamycin Aesculin Azide Agar, 50 kg (US only size)

Reference Material

NCM0198 - Kanamycin Aesculin Azide Agar, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0198 - Kanamycin Aesculin Azide Agar, Technical Specifications Sheet [English]

For additional information or questions about product SDS documents, please contact Neogen at

Please check with your local representative for availability of these products within your region.