Product number: NCM0221

Iron Sulphite Agar is a medium for the detection of thermophilic anaerobic organisms causing sulphide spoilage in food, and is not intended for use in the diagnosis of disease or other conditions in humans.

This formulation is a modification of Cameron Sulphite Agar, which was developed by the National Canners Association of America (now the Grocery Manufacturers Association). Iron Sulphite Agar has a reduced concentration of sodium sulphite to allow improved detection of some strains of Clostridium sporogenes. Beerens, and later Mossel, demonstrated that some strains of C. sporogenes would not tolerate sodium sulphite levels of 0.1%. Mossel further observed that reducing sulphite content to 0.05% improved detection of these strains.

Tryptone provides nitrogen and other nutrients necessary to support bacterial growth. The presence of sulphite reducing bacteria is indicated by the formation of black colonies. These colonies form when bacteria reduce sulphite to sulphide, which reacts with iron (III) citrate to yield a black precipitate.


Formula Liter
Tryptone 10.0 g/L
Sodium Sulphite 0.5 g/L
Iron (III) Citrate 0.5 g/L
Agar 12.0 g/L

Final pH: 7.1 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 23 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C.

Product was previously known as LabM Product: LAB222 Iron Sulphite Agar

Order Details

Product # Product Description
NCM0221A Iron Sulphite Agar, 500 g
NCM0221B Iron Sulphite Agar, 5 kg
NCM0221C Iron Sulphite Agar, 10 kg
NCM0221D Iron Sulphite Agar, 25 kg (European only size)
NCM0221E Iron Sulphite Agar, 50 kg (US only size)

Reference Material

NCM0221 - Iron Sulphite Agar, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Hungarian] [Italian] [Spanish, Mexico] [Swedish]
NCM0221 - Iron Sulphite Agar, Technical Specifications Sheet [English]

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