Product number: NCM0006

Hektoen Enteric Agar is used for the isolation and differentiation of enteric pathogens. Hektoen Enteric Agar is not intended for use in the diagnosis of disease or other conditions in humans.

Hektoen Enteric Agar was developed in 1967 by King and Metzger. Compared to other enteric differentiating media commonly used, Hektoen Enteric Agar increased the isolation rate of Salmonella spp. and Shigella spp. This was accomplished by increasing the carbohydrate and peptone content of the medium in order to counteract the inhibitory effects of bile salts and indicators. King and Metzger formulated a medium that slightly inhibited growth of Salmonella and Shigella, while inhibiting Gram-positive microorganisms.

Enzymatic Digest of Animal Tissue provides nitrogen, carbon, and amino acids required for organism growth. Yeast Extract is a vitamin source. Bile Salts Mixture and Acid Fuchsin inhibit Gram-positive organisms. Lactose, Sucrose, and Salicin are fermentable carbohydrates. Sodium Chloride maintains the osmotic balance of the medium. Ferric Ammonium Citrate, a source of iron, allows the detection of hydrogen sulfide (H2S) produced from Sodium Thiosulfate. H2S-positive colonies have black centers. Bromothymol Blue is added as the pH indicator. Agar is the solidifying agent.


Formula Liter
Enzymatic Digest of Animal Tissue 16.5 g/L
Yeast Extract 3.0 g/L
Bile Salts Mixture 4.5 g/L
Lactose 12.0 g/L
Sucrose 12.0 g/L
Salicin 2.0 g/L
Sodium Chloride 5.0 g/L
Sodium Thiosulfate 5.0 g/L
Ferric Ammonium Citrate 1.5 g/L
Bromthymol Blue 0.065 g/L
Acid Fuchsin 0.1 g/L
Agar 13.5 g/L

pH: 7.6 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 75 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.

Product was previously known as Acumedia Product: 7138 Hektoen Enteric Agar; and LabM Product: LAB110 Hektoen Enteric Medium

Order Details

Product # Product Description
NCM0006A Hektoen Enteric (HE) Agar, 500 g
NCM0006B Hektoen Enteric (HE) Agar, 5 kg
NCM0006C Hektoen Enteric (HE) Agar, 10 kg
NCM0006D Hektoen Enteric (HE) Agar, 25 kg (European only size)
NCM0006E Hektoen Enteric (HE) Agar, 50 kg (US only size)

This product is also available with a CE mark but with restricted sales geographically. Please click here for product details.

Reference Material

NCM0006 - Hektoen Enteric (HE) Agar, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0006 - Hektoen Enteric (HE) Agar, Technical Specifications Sheet [English]

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