Product number: NCM1003

Salmonella ABC Medium is for the isolation of Salmonella spp, and is not intended for use in the diagnosis of disease or other conditions in humans.

Salmonella spp. can be differentiated from other members of the family Enterobacteriaceae by their ability to produce α-galactosidase in the absence of β-galactosidase. This medium, developed for the isolation of Salmonella spp. from food and clinical samples, utilizes a dual chromogen system to visualize these enzyme activities. This medium will also detect Salmonella typhi and paratyphi.

Formula Liter
Beef Extract 5.0 g/L
Peptone 5.0 g/L
Sodium Citrate 8.5 g/L
Sodium Deoxycholate 5.0 g/L
Agar 12.0 g/L
X-α-Gal 0.08 g/L
CHE-β-Gal 0.3 g/L
Ferric Ammonium Citrate 0.5 g/L
IPTG 0.03 g/L

Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 36.5 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Cool to 45-50°C.

Product was previously known as LabM Product: HAL001 Harlequin® Salmonella ABC Medium

Order Details

Product # Product Description
NCM1003A Harlequin Salmonella ABC Medium, 500 g
NCM1003B Harlequin Salmonella ABC Medium, 5 kg
NCM1003C Harlequin Salmonella ABC Medium, 10 kg
NCM1003D Harlequin Salmonella ABC Medium, 25 kg (European only size)
NCM1003E Harlequin Salmonella ABC Medium, 50 kg (US only size)

Reference Material

NCM1003 - Harlequin Salmonella ABC Medium, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Hungarian] [Italian] [Spanish, Mexico] [Swedish]
NCM1003 - Harlequin Salmonella ABC Medium, Technical Specifications Sheet [English]
HAL001 - Harlequin Salmonella ABC Medium, Product Information Sheet [English]

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