Product number: NCM0152

Glucose OF Medium is used for the confirmation of Enterobacteriaceae in foods according to ISO 21528-1&2:2017, and is not intended for use in the diagnosis of disease or other conditions in humans.

Enzymatic digest of casein provides nitrogen, vitamins, minerals and amino acids essential for growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Dipotassium phosphate acts as a buffer system. Glucose is the fermentable carbohydrate providing carbon and energy. Bromothymol blue is the pH indicator.

Colonies of presumptive Enterobacteriaceae should be confirmed by means of tests for the fermentation of glucose and the presence of oxidase. If the colonies are oxidase-negative and glucose-positive, the sample shall be regarded as being positive for Enterobacteriaceae. ISO 21528 :2017 recommends this medium to confirm the presumptive colonies of Enterobacteriaceae by fermentation test.


Formula Liter
Enzymatic Digest of Casein 2.0 g/L
Dipotassium Hydrogen Phosphate 0.3 g/L
Glucose 10.0 g/L
Sodium Chloride 5.0 g/L
Bromthymol Blue 0.08 g/L
Agar 4.0 g/L

Final pH: 6.8 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Dissolve 21.4 grams of the medium into one liter of purified water.
  2. Heat with frequent agitation to completely dissolve the medium if necessary.
  3. Autoclave at 121ºC for 15 minutes.
  4. Cool to 45-50ºC.

Product was previously known as LabM Product: LAB227

Order Details

Product # Product Description
NCM0152A Glucose OF Medium, 500 g
NCM0152B Glucose OF Medium, 5 kg
NCM0152C Glucose OF Medium, 10 kg
NCM0152D Glucose OF Medium, 25 kg (European only size)
NCM0152E Glucose OF Medium, 50 kg (US only size)

Reference Material

NCM0152 - Glucose OF Medium, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Hungarian] [Italian] [Spanish, Mexico] [Swedish]
NCM0152 - Glucose OF Medium, Technical Specifications Sheet [English]

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