Product number: NCM0050

Fraser Broth is used with ferric ammonium citrate for the selective enrichment of Listeria species and is not intended for use in the diagnosis of disease or other conditions in humans.

Fraser Broth is a secondary enrichment broth for the isolation of Listeria spp. formulated according to ISO 11290. The selective components acriflavine and nalidixic acid are blended into the base powder and the ferric ammonium citrate (X211 or 7984) is added to the tempered broth after sterilization.

 

Formula Liter
Peptone 15.0 g/L
Yeast Extract 5.0 g/L
Esculin 1.0 g/L
Disodium Hydrogen Phosphate 9.6 g/L
Potassium Dihydrogen Phosphate 1.35 g/L
Sodium Chloride 20.0 g/L
Lithium Chloride 3.0 g/L
Acriflavine 0.025 g/L
Nalidixic Acid 0.02 g/L

pH: 7.2 ± 0.2 at 25°C
*equivalent to Disodium Hydrogen Phosphate Dihydrate 12.0 g/l
Formula may be adjusted and/or supplemented as required to meet performance specifications.
Ferric Ammonium Citrate Supplement (X211) or Fraser broth base supplement (7984) may be used

Preparation

  1. Dissolve 55 grams of the medium in 1 liter of purified water.
  2. Mix thoroughly
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C.
  5. Aseptically add 2 vials of Ferric Ammonium Citrate Supplement (X211) or 2 vials of Fraser Broth Base Supplement (7984) and mix thoroughly before dispensing.

Product was previously known as Acumedia Product: 7502 Fraser Broth Base; and LabM Product: LAB212 Fraser Broth PLUS

Order Details

Product # Product Description
NCM0050A Fraser Broth, 500 g
NCM0050B Fraser Broth, 5 kg
NCM0050C Fraser Broth, 10 kg
NCM0050D Fraser Broth, 25 kg (European only size)
NCM0050E Fraser Broth, 50 kg (US only size)

Reference Material

NCM0050 - Fraser Broth, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0050 - Fraser Broth, Technical Specifications Sheet [English]

For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.

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