Product number: NCM2020

Fastidious Anaerobe Agar is used for the cultivation of anaerobic microorganisms.

A primary isolation medium capable of growing most clinically significant anaerobes. Developed by Lab M (Neogen Corporation), comparisons have shown this medium to be superior to other formulations as a primary isolation medium for fastidious organisms. The peptones included have been chosen for maximum growth stimulation. Starch and sodium bicarbonate act as de-toxification agents while hemin encourages pigment production in Porphyromonas melaninogenicus. Specific growth promoting agents are Cysteine for Fusobacterium necrophorum, Propionibacterium acne and Bacteriodes fragilis, arginine for Eubacterium spp. soluble pyrophosphate for Porph. gingivalis and Porph. asaccharolyticus. Pyruvate helps neutralize hydrogen peroxide and is also utilized by Veillionella spp. as an energy source. Vitamin K and sodium succinate provide essential growth factors for some anaerobes as does the 0.1% glucose. The low level of glucose prevents the production of high levels of acids and alcohols which would inhibit colonial development.

 

Formula Liter
Peptone Mix 23.0 g/L
Sodium Chloride 5.0 g/L
Soluble Starch 1.0 g/L
Agar 12.0 g/L
Sodium Bicarbonate 0.4 g/L
Glucose 1.0 g/L
Sodium Pyruvate 1.0 g/L
Cysteine HCl Monohydrate 0.5 g/L
Hemin 0.01 g/L
Vitamin K 0.001 g/L
L-Arginine 1.0 g/L
Soluble Pyrophosphate 0.25 g/L
Sodium Succinate 0.5 g/L

Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Preparation

  1. Suspend 46 grams of the medium and add to 1 liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Prepare 7% blood agar by aseptically adding the appropriate volume of sterile defibrinated blood to melted sterile agar medium, cooled to 45-50°C.

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Order Details

Product # Product Description
NCM2020A Fastidious Anaerobe Agar CE, 500 g
NCM2020B Fastidious Anaerobe Agar CE, 5 kg
NCM2020C Fastidious Anaerobe Agar CE, 10 kg
NCM2020D Fastidious Anaerobe Agar CE, 25 kg (European only size)

Reference Material

NCM2020 - Fastidious Anaerobe Agar CE, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Hungarian] [Italian] [Spanish, Mexico] [Swedish]
NCM2020 - Fastidious Anaerobe Agar CE, Technical Specifications Sheet [English]

For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.

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