Product number: NCM0105

Eosin Methylene Blue Agar Levine is used for the isolation and differentiation of Gram-negative enteric bacilli and is not intended for use in the diagnosis of disease or other conditions in humans.

This medium was introduced in 1916 by Holt-Harris and Teague to differentiate Escherichia spp. and Aerobacter spp. It was modified by Levine in 1918 who removed sucrose from the formula and increased the lactose content. The distinctive metallic sheen produced by E. coli on this medium is due to acid production resulting in an amide bonding between the eosin and methylene blue, other coliforms do not produce enough acid to cause this reaction. Eosin inhibits most Gram-positive organisms. The prepared medium is sensitive to light.


Formula Liter
Peptone 10.0 g/L
Lactose 10.0 g/L
Dipotassium Hydrogen Phosphate 2.0 g/L
Eosin Yellow 0.4 g/L
Methylene Blue 0.065 g/L
Agar 15.0 g/L

pH: 6.8 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 37.5 grams of the medium and add to 1 liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C and agitate gently to ensure uniform distribution of the flocculant precipitate which is a feature of this medium before pouring into Petri dishes.

Product was previously known as Acumedia Product: 7103 Eosin Methylene Blue Agar, Levine; and LabM Product: LAB061 Eosin Methylene Blue Agar (Levine)

Order Details

Product # Product Description
NCM0105A Eosin Methylene Blue (EMB) Agar Levine, 500 g
NCM0105B Eosin Methylene Blue (EMB) Agar Levine, 5 kg
NCM0105C Eosin Methylene Blue (EMB) Agar Levine, 10 kg
NCM0105D Eosin Methylene Blue (EMB) Agar Levine, 25 kg (European only size)
NCM0105E Eosin Methylene Blue (EMB) Agar Levine, 50 kg (US only size)

Reference Material

NCM0105 - Eosin Methylene Blue (EMB) Agar, Levine, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0105 - Eosin Methylene Blue (EMB) Agar, Levine, Technical Specifications Sheet [English]

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