Product number: NCM0057

Enterobacteria Enrichment (EE) Broth Mossel is used for the cultivation and enrichment of Enterobacteriaceae in food. Enterobacteria Enrichment (EE) Broth Mossel, conforms to Harmonized USP/EP/JP Requirements and is not intended for use in the diagnosis of disease or other conditions in humans.

Enterobacteria Enrichment Broth Mossel was developed by Mossel, Visser, and Cornelissen to facilitate the growth of Enterobacteriaceae. This medium contains dextrose to enhance the growth of E. coli and Salmonella spp., particularly in food samples. Nuisance organisms are suppressed by the addition of Ox Bile and Brilliant Green.

Enterobacteria Enrichment Broth Mossel is used as an enrichment broth, providing a rich environment for the recovery of damaged or injured cells. Enterobacteriaceae organisms can be injured in food-processing procedures, including exposure to low temperature, sub-marginal heat, drying, radiation, preservatives, or sanitizers. The enumeration of Enterobacteriaceae is an important measure of the sanitary condition of food.

EE Broth, Mossel complies with the specifications of the Eiprodukte-Verodnung (German Egg Product Regulations) and conforms to Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EU), and Japanese Pharmacopoeia (JP).


Formula Liter
Dehydrated Ox Bile 20.0 g/L
Enzymatic Digest of Gelatin 10.0 g/L
Disodium Hydrogen Phosphate Dihydrate 8.0 g/L
Glucose Monohydrate 5.0 g/L
Potassium DiHydrogen Phosphate 2.0 g/L
Brilliant Green 0.015 g/L

pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 45g of the medium in one liter of purified water.
  2. Heat at 100°C for 30 minutes to completely dissolve the medium.
  3. Cool rapidly in cold water.

Product was previously known as Acumedia Product: 7603 EE Broth, Mossel; and LabM Products: LAB091 EE Broth - Enterobacteriaceae Enrichment and HP003 Enterobacteria Enrichment Broth - Mossel (USP/EP/JP)

Order Details

Product # Product Description
NCM0057A Enterobacteria Enrichment (EE) Broth Mossel, 500 g
NCM0057B Enterobacteria Enrichment (EE) Broth Mossel, 5 kg
NCM0057C Enterobacteria Enrichment (EE) Broth Mossel, 10 kg
NCM0057D Enterobacteria Enrichment (EE) Broth Mossel, 25 kg (European only size)
NCM0057E Enterobacteria Enrichment (EE) Broth Mossel, 50 kg (US only size)

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Reference Material

NCM0057 - Enterobacteriaceae Enrichment (EE) Broth Mossel, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0057 - Enterobacteriaceae Enrichment (EE) Broth Mossel, Technical Specifications Sheet [English]

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