Product number: NCM0065

EC Broth is used for the detection of coliform bacteria at 37°C and Escherichia coli at 44.5°C. EC Broth is not intended for use in the diagnosis of disease or other conditions in humans.

EC Broth (Escherichia coli Broth) is a selective enrichment broth designed for the isolation of coliforms, including E. coli, from water and food samples. It was the recommended medium of the American Public Health Association (APHA) and the AOAC.

EC Broth is made selective for coliforms by the inclusion of Bile Salts in the dehydrated medium. The selective nature of this medium ensures that the growth of non-coliform bacteria is minimized. The medium is buffered by the addition of potassium phosphates and osmotically balanced by sodium chloride. The medium is used at 37°C for coliform organisms and 44.5°C is recommended for the isolation E. coli.


Formula Liter
Tryptone 20.0 g/L
Lactose 5.0 g/L
Bile Salts 1.5 g/L
Dipotassium Phosphate 4.0 g/L
Potassium Diphosphate 1.5 g/L
Sodium Chloride 5.0 g/L

Final pH: 6.9 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Dissolve 37 grams of the medium in 1000 mL of purified water.
  2. Mix thoroughly.
  3. Dispense into tubes of appropriate volume and, where applicable, add inverted Durham tubes.
  4. Sterilize by autoclaving at 121°C for 15 minutes.

Product was previously known as Acumedia Product: 7206 EC Broth; and LabM Product: LAB171 E.C. Medium

Order Details

Product # Product Description
NCM0065A EC Broth, 500 g
NCM0065B EC Broth, 5 kg
NCM0065C EC Broth, 10 kg
NCM0065D EC Broth, 25 kg (European only size)
NCM0065E EC Broth, 50 kg (US only size)

Reference Material

NCM0065 - EC Broth, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0065 - EC Broth, Technical Specifications Sheet [English]

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