Product number: NCM0082

Dichloran Rose Bengal Chloramphenicol Agar is for the enumeration of yeasts and is not intended for use in the diagnosis of disease or other conditions in humans.

Developed with reference to ISO 21527-1:2008 for the enumeration of yeasts and molds in food and animal products, this medium is tested to the performance requirements of the standard. Used for the enumeration of viable yeasts and molds in products with a water activity of greater than 0.95 such as eggs, meat, some dairy products, fresh pastes, fruit and vegetables, DRBC Agar is designed to suppress the colonial growth of ‘spreader’ molds and in doing so allow easier performance of the colony count technique on yeasts and molds.

The use of the anti-fungal agent, dichloran, restricts spreading of mucoraceous fungi and restricts the colony size of other genera. Rose bengal also assists in the reduction of colony sizes and is selective against bacteria. Additional selectivity against bacterial growth is achieved through both the low pH of the medium and the incorporation of the heat-stable antibiotic Chloramphenicol. Glucose is incorporated as the fermentable carbohydrate source, with an enzymatic digest of animal & plant tissues providing the essential vitamins, minerals, amino acids, nitrogen and carbon.

 

Formula Liter
Enzymatic Digest of Animal & Plant Tissue 5.0 g/L
D-Glucose 10.0 g/L
Potassium Dihydrogen Phosphate 1.0 g/L
Magnesium Sulphate 0.5 g/L
Dichloran 0.002 g/L
Chloramphenicol 0.1 g/L
Rose Bengal 0.025 g/L
Agar 15.0 g/L

Final pH: 5.6 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Preparation

  1. Suspend 31.7 grams of medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C.

Product was previously known as LAB217 DRBC Agar - Dichloran Rose Bengal Chloramphenicol Agar

Order Details

Product # Product Description
NCM0082A DRBC Agar (ISO), 500 g
NCM0082B DRBC Agar (ISO), 5 kg
NCM0082C DRBC Agar (ISO), 10 kg
NCM0082D DRBC Agar (ISO), 25 kg (European only size)
NCM0082E DRBC Agar (ISO), 50 kg (US only size)

Reference Material

LAB217 - DRBC Agar (ISO), Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Hungarian] [Italian] [Spanish, Mexico] [Swedish]
NCM0082 - DRBC Agar (ISO), Technical Specifications Sheet [English]

For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.

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