Product number: NCM0081

Dichloran Glycerol (DG-18) Agar Base is used for the selective isolation and enumeration of yeasts and molds from foods in a laboratory setting. Dichloran Glycerol (DG-18) Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.

Dichloran Glycerol (DG-18) Agar Base is used for the enumeration of viable osmophilic yeasts and xerophilic molds in food or animal feed products with a water activity of less than or equal to 0.95. This includes such foods as dry fruits, jams, cakes, dried meat, salted fish, grains, cereals, flours, nuts, spices, condiments and some animal feeds. This medium is not suitable for the examination of dehydrated products with a water activity of greater than 0.95, Dichloran Rose Bengal Chloramphenicol Agar (NCM0082 or NCM0029) should be used.

The reduction in water activity in this medium is achieved by the addition of glycerol at approximately 18% and this is very important as many yeast and molds require a low water activity to enhance growth and colony development. The medium also contains the antifungal agent dichloran, which restricts the spreading of mucoraceous fungi and restricts the colony size of other genera making colony counting an easier task.

Additional selectivity against bacterial growth is achieved by the incorporation of the heat-stable antibiotic Chloramphenicol. Glucose is incorporated as the fermentable carbohydrate source, with casein enzymatic digest providing the essential vitamins, minerals, amino acids, nitrogen and carbon.

 

Formula Liter
Casein Enzymatic Digest 5.0 g/L
D-Glucose 10.0 g/L
Potassium Dihydrogen Phosphate 1.0 g/L
Magnesium Sulfate 0.5 g/L
Dichloran 0.002 g/L
Chloramphenicol 0.1 g/L
Agar 15.0 g/L

pH: 5.6 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Preparation

  1. Suspend 31.6 g of medium and 220 grams of glycerol in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. DO NOT OVERHEAT
  5. Cool to 45-50°C.

Product was previously known as Acumedia Product: 7592 Dichloran Glycerol (DG-18) Agar Base; and LabM Product: LAB218 DG18 Agar - Dichloran (18%) Glycerol Agar

Order Details

Product # Product Description
NCM0081A Dichloran Glycerol (DG-18) Agar Base, 500 g
NCM0081B Dichloran Glycerol (DG-18) Agar Base, 5 kg
NCM0081C Dichloran Glycerol (DG-18) Agar Base, 10 kg
NCM0081D Dichloran Glycerol (DG-18) Agar Base, 25 kg (European only size)
NCM0081E Dichloran Glycerol (DG-18) Agar Base, 50 kg (US only size)

Reference Material

7592 - Dichloran Glycerol (DG-18) Agar Base, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Hungarian] [Italian] [Spanish, Mexico] [Swedish]
NCM0081 - Dichloran Glycerol (DG-18) Agar Base, Technical Specifications Sheet [English]

For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.

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