Product number: NCM0121

D/E Neutralizing Broth is used to neutralize and determine the bactericidal activity of antiseptics and disinfectants. D/E Neutralizing Broth without Tween is not intended for use in the diagnosis of disease or other conditions in humans.

D/E Neutralizing Broth was developed by Dey and Engley to neutralize a broad spectrum of disinfectants and preservative antimicrobial chemicals, including quaternary ammonium compounds, phenolics, iodine, chlorine preparations, mercurials, formaldehyde, and glutaraldehyde. D/E Neutralizing media neutralize higher concentrations of residual antimicrobials when compared with other standard neutralizing formulations, such as Letheen media, Thioglycollate media, and Neutralizing Buffer.

Total neutralization of disinfectants is critical. Disinfectant residues can result in a false-negative (no-growth) test. D/E Neutralizing Broth without Tween effectively neutralizes the inhibitor action of disinfectant carryover, allowing differentiation between bacteriostasis and the true bactericidal action of disinfectant chemicals. This is a critical characteristic to consider when evaluating a disinfectant. D/E Neutralizing Broth without Tween is recommended for use in disinfectant evaluations, environmental sampling (swab and contact plate methods), and testing of water-miscible cosmetics.

 

Formula Liter
Enzymatic Digest of Casein 5.0 g/L
Yeast Extract 2.5 g/L
Dextrose (Glucose) 10.0 g/L
Sodium Thioglycollate 1.0 g/L
Sodium Thiosulfate 6.0 g/L
Sodium Bisulfite 2.5 g/L
Lecithin 7.0 g/L
Bromocresol Purple 0.02 g/L

Final pH: 7.6 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Supplement

Polysorbate 80 (7992), 5g/L

Preparation

  1. Dissolve 34 g of the medium and 5 grams of Tween (Polysorbate) 80 in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.

Product was previously known as Acumedia Product: 7562 DE Neutralizing Broth (no tween)

Order Details

Product # Product Description
NCM0121A D/E Neutralizing Broth without Tween, 500 g
NCM0121B D/E Neutralizing Broth without Tween, 5 kg
NCM0121C D/E Neutralizing Broth without Tween, 10 kg
NCM0121D D/E Neutralizing Broth without Tween, 25 kg (European only size)
NCM0121E D/E Neutralizing Broth without Tween, 50 kg (US only size)

Reference Material

NCM0121 - D/E Neutralizing Broth without Tween, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Hungarian] [Italian] [Spanish, Mexico] [Swedish]
NCM0121 - D/E Neutralizing Broth without Tween, Technical Specifications Sheet [English]

For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.

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