Product number: NCM0047

D/E Neutralizing Broth is used to neutralize and determine the bactericidal activity of antiseptics and disinfectants. D/E Neutralizing Broth is not intended for use in the diagnosis of disease or other conditions in humans.

D/E Neutralizing Broth was developed by Dey and Engley to neutralize a broad spectrum of disinfectants and preservative antimicrobial chemicals, including quaternary ammonium compounds, phenolics, iodine, chlorine preparations, mercurials, formaldehyde, and glutaraldehyde. D/E Neutralizing media neutralize higher concentrations of residual antimicrobials when compared with other standard neutralizing formulations, such as Letheen media, Thioglycolate media, and Neutralizing Buffer.

Total neutralization of disinfectants is critical. Disinfectant residues can result in a false-negative (no-growth) test. D/E Neutralizing Broth w/Tween effectively neutralizes the inhibitor action of disinfectant carryover, allowing differentiation between bacteriostasis and the true bactericidal action of disinfectant chemicals. This is a critical characteristic to consider when evaluating a disinfectant. D/E Neutralizing Broth w/Tween is recommended for use in disinfectant evaluations, environmental sampling (swab and contact plate methods), and testing of water-miscible cosmetics.


Formula Liter
Dextrose 10.0 g/L
Lecithin 7.0 g/L
Sodium Thiosulfate 6.0 g/L
Polysorbate 80 5.0 g/L
Enzymatic Digest of Casein 5.0 g/L
Yeast Extract 2.5 g/L
Sodium Bisulfite 2.5 g/L
Sodium Thioglycolate 1.0 g/L
Bromocresol Purple 0.02 g/L

Final pH: 7.6 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Dissolve 39 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.

Product was previously known as Acumedia Product: 7705 D/E Neutralizing Broth with Tween; and LabM Product: LAB187 D/E Neutralising Broth (Dey & Engley)

Order Details

Product # Product Description
NCM0047A D/E Neutralizing Broth, 500 g
NCM0047B D/E Neutralizing Broth, 5 kg
NCM0047C D/E Neutralizing Broth, 10 kg
NCM0047D D/E Neutralizing Broth, 25 kg (European only size)
NCM0047E D/E Neutralizing Broth, 50 kg (US only size)

Reference Material

NCM0047 - D/E Neutralizing Broth, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Hungarian] [Italian] [Spanish, Mexico] [Swedish]
NCM0047 - D/E Neutralizing Broth, Technical Specifications Sheet [English]

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