Product number: NCM0009

D/E Neutralizing Agar with Tween is used for the isolation of microorganisms from sanitized environmental surfaces and is not intended for use in the diagnosis of disease or other conditions in humans.

D/E Neutralizing Agar with Tween was developed by Dey and Engley to neutralize a broad spectrum of disinfectants and preservative antimicrobial chemicals, including quaternary ammonium compounds, phenolics, iodine, chlorine preparations, mercurials, formaldehyde, and glutaraldehyde. D/E Neutralizing media neutralize higher concentrations of residual antimicrobials when compared with other standard neutralizing formulations, such as Letheen media, Thioglycollate media, and Neutralizing Buffer.

Total neutralization of disinfectants is critical. Disinfectant residues can result in a false negative (nogrowth) test. D/E Neutralizing Agar effectively neutralizes the inhibitory action of disinfectant carryover, allowing differentiation between bacteriostasis and true bactericidal action of disinfectant chemicals. This is a critical characteristic to consider when evaluating a disinfectant. D/E Neutralizing Agar with Tween is recommended for use in disinfectant evaluations, environmental sampling (swab and contact plate methods), and testing of water-miscible cosmetics.

 

Formula Liter
Enzymatic Digest of Casein 5.0 g/L
Yeast Extract 2.5 g/L
Dextrose 10.0 g/L
Sodium Thioglycollate 1.0 g/L
Sodium Thiosulfate 6.0 g/L
Sodium Bisulfite 2.5 g/L
Polysorbate 80 (Tween) 5.0 g/L
Lecithin 7.0 g/L
Bromcresol Purple 0.02 g/L
Agar 15.0 g/L

Final pH: 7.6 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Preparation

  1. Suspend 54 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Product was previously known as Acumedia Product: 7375 D/E Neutralising Agar; and LabM Product: LAB188 D/E Neutralising Agar (Dey & Engley)

Order Details

Product # Product Description
NCM0009A D/E Neutralizing Agar with Tween, 500 g
NCM0009B D/E Neutralizing Agar with Tween, 5 kg
NCM0009C D/E Neutralizing Agar with Tween, 10 kg
NCM0009D D/E Neutralizing Agar with Tween, 25 kg (European only size)
NCM0009E D/E Neutralizing Agar with Tween, 50 kg (US only size)

Reference Material

7375 - D/E Neutralizing Agar, Product Information Sheet [English] [Portuguese]
7375 - D/E Neutralizing Agar, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0009 - D/E Neutralizing Agar, Technical Specifications Sheet [English]

For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.

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