Product number: NCM0034

Cystine Lactose Electrolyte Deficient (CLED) Agar is used for the differentiation and enumeration of microorganisms in urine.

A medium for urine culture where the absence of electrolytes inhibits the swarming of Proteus spp. Cystine is added for the benefit of those organisms which have a specific cystine requirement. Differentiation of lactose and non-lactose fermenters is achieved using bromothymol blue as pH indicator. This medium supports the growth of Streptococcus pyogenes and most other fastidious organisms that do not require blood.

 

Formula Liter
Balanced Peptone 4.0 g/L
Beef Extract 3.0 g/L
Tryptone 4.0 g/L
Lactose 10.0 g/L
L-Cystine 0.128 g/L
Bromothymol Blue 0.02 g/L
Agar 15.0 g/L

Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Preparation

  1. Suspend 36 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C.

Product was previously known as Acumedia Product: 7122 CLED Agar; and LabM Product: LAB041 CLED Medium (Mackey and Sandys)

Order Details

Product # Product Description
NCM0034A Cystine Lactose Electrolyte Deficient (CLED) Agar, 500 g
NCM0034B Cystine Lactose Electrolyte Deficient (CLED) Agar, 5 kg
NCM0034C Cystine Lactose Electrolyte Deficient (CLED) Agar, 10 kg
NCM0034D Cystine Lactose Electrolyte Deficient (CLED) Agar, 25 kg (European only size)
NCM0034E Cystine Lactose Electrolyte Deficient (CLED) Agar, 50 kg (US only size)

This product is also available with a CE mark but with restricted sales geographically. Please click here for product details.

Reference Material

NCM0034 - Cystine Lactose Electrolyte Deficient (CLED) Agar, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0034 - Cystine Lactose Electrolyte Deficient (CLED) Agar, Technical Specifications Sheet [English]
LAB041CE - Cystine Lactose Electrolyte Deficient (CLED) Agar, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Hungarian] [Italian] [Spanish, Mexico] [Swedish]

For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.

Please check with your local representative for availability of these products within your region.