Product number: NCM2023

Columbia Blood Agar Base is used with blood for the isolation and cultivation of a wide variety of fastidious microorganisms.

A modification of the original Columbia Agar base formulation, Columbia Blood Agar Base provides a medium that is suitable for use with both defibrinated horse or sheep blood. The peptone mixture provides a source of nitrogen, essential vitamins and amino acids. The starch provides a carbon source and sodium chloride maintains osmotic balance. Originally described as a general purpose nutritious agar base by Ellner et al. Columbia Agar is more frequently used when enriched by the addition of sterile blood. The medium is suitable for supporting the growth and determining hemolytic reactions of a variety of microorganisms.


Formula Liter
Columbia peptone mixture 25.1 g/L
Soluble starch 1.0 g/L
Sodium chloride 5.0 g/L
Agar 12.0 g/L

Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 43.1 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C and add 5-7% sterile defibrinated horse or sheep blood.
  5. Mix well before dispensing into sterile Petri dishes.

Product is only available in certain countries. Please contact your account manager if you require furthur details.

Order Details

Product # Product Description
NCM2023A Columbia Blood Agar Base CE, 500 g
NCM2023B Columbia Blood Agar Base CE, 5 kg
NCM2023C Columbia Blood Agar Base CE, 10 kg
NCM2023D Columbia Blood Agar Base CE, 25 kg (European only size)

Reference Material

NCM2023 - Columbia Agar II Base CE, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Hungarian] [Italian] [Spanish, Mexico] [Swedish]
NCM2023 - Columbia Agar II Base CE, Technical Specifications Sheet [EU English]

For additional information or questions about product SDS documents, please contact Neogen at

Please check with your local representative for availability of these products within your region.