Product number: NCM0031

Columbia Blood Agar Base is used with blood for the isolation and cultivation of a wide variety of fastidious microorganisms. Columbia Blood Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.

A modification of the original Columbia Agar base formulation, Columbia Blood Agar Base provides a medium that is suitable for use with both defibrinated horse or sheep blood. The peptone mixture provides a source of nitrogen, essential vitamins and amino acids. The starch provides a carbon source and sodium chloride maintains osmotic balance. Originally described as a general purpose nutritious agar base by Ellner et al. Columbia Agar is more frequently used when enriched by the addition of sterile blood. The medium is suitable for supporting the growth and determining hemolytic reactions of a variety of microorganisms.


Formula Liter
Columbia peptone mixture 25.1 g/L
Soluble starch 1.0 g/L
Sodium chloride 5.0 g/L
Agar 12.0 g/L

Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 43.1 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C and add 5-7% sterile defibrinated horse or sheep blood.
  5. Mix well before dispensing into sterile Petri dishes.

Product was previously known as Acumedia Product: 7125 Columbia Blood Agar Base; and LabM Product: LAB215 Columbia II Agar Base

Order Details

Product # Product Description
NCM0031A Columbia Blood Agar Base, 500 g
NCM0031B Columbia Blood Agar Base, 5 kg
NCM0031C Columbia Blood Agar Base, 10 kg
NCM0031D Columbia Blood Agar Base, 25 kg (European only size)
NCM0031E Columbia Blood Agar Base, 50 kg (US only size)

This product is also available with a CE mark but with restricted sales geographically. Please click here for product details.

Reference Material

NCM0031 - Columbia Blood Agar Base, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0031 - Columbia Blood Agar Base, Technical Specifications Sheet [English]

For additional information or questions about product SDS documents, please contact Neogen at

Please check with your local representative for availability of these products within your region.