Product number: NCM2010

Columbia Agar Base is used with blood for the isolation and cultivation of a wide variety of fastidious microorganisms.

A modification of the original Columbia Agar base formulation, Columbia Agar Base provides a medium that is suitable for use with both defibrinated horse and sheep blood. The peptone mixture provides a source of nitrogen, essential vitamins and amino acids. The starch provides a carbon source and sodium chloride maintains osmotic balance. Originally described as a general purpose nutritious agar base by Ellner et al. Columbia Agar is more frequently used when enriched by the addition of sterile blood. The medium is suitable for supporting the growth and determining hemolytic reactions of a variety of microorganisms


Formula Liter
Columbia Peptone Mixture 23.0 g/L
Corn Starch 1.0 g/L
Sodium Chloride 5.0 g/L
Agar 12.0 g/L

Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 41 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Prepare 5 to 10% blood agar by adding appropriate volume of sterile defribrinated horse or sheep blood to melted sterile agar medium, cooled to 45-50°C.

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Order Details

Product # Product Description
NCM2010A Columbia Agar Base CE, 500 g
NCM2010B Columbia Agar Base CE, 5 kg
NCM2010C Columbia Agar Base CE, 10 kg
NCM2010D Columbia Agar Base CE, 25 kg (European only size)

Reference Material

NCM2010 - Columbia Agar Base CE, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Hungarian] [Italian] [Spanish, Mexico] [Swedish]
NCM2010 - Columbia Agar Base CE, Technical Specifications Sheet [English]

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