Product number: NCM0013

Columbia Agar is used with or without blood for the isolation and cultivation of a wide variety of fastidious microorganisms. Conforms to Harmonized USP/EP/JP Requirements. Columbia Agar is not intended for use in the diagnosis of disease or other conditions in humans.

A medium recommended by the Harmonized USP/EP/JP for isolation and identification of Clostridia from non-sterile products. Conforms to Harmonized USP/EP/JP performance specification. Originally described as a general purpose nutritious agar base by Ellner et al. at Columbia University that can be enriched by the addition of sterile blood. The peptone mixture and yeast extract provide a source of nitrogen, essential vitamins and amino acids. The starch provides a carbon source and sodium chloride maintains osmotic balance. The Harmonized European Pharmacopoeia states that where necessary, gentamicin sulfate at a concentration of 20mg/L can be added after sterilization to reduce the growth of non-target organisms. According to the Harmonized European Pharmacopoeia, Reinforced Medium for Clostridia is used as a selective enrichment broth, with subculture performed onto Columbia Agar.


Formula Liter
Pancreatic Digest of Casein 10.0 g/L
Meat Peptic Digest 5.0 g/L
Heart Pancreatic Digest 3.0 g/L
Yeast Extract 5.0 g/L
Maize Starch 1.0 g/L
Sodium Chloride 5.0 g/L
Agar 12.0 g/L

Final pH: 7.3 ± 0.2 at 25 C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Dissolve 41 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool 45-50°C.

Product was previously known as Acumedia Product: 7734 Columbia Agar; and LabM Product: HP012 Columbia Agar (USP/EP/JP)

Order Details

Product # Product Description
NCM0013A Columbia Agar, 500 g
NCM0013B Columbia Agar, 5 kg
NCM0013C Columbia Agar, 10 kg
NCM0013D Columbia Agar, 25 kg (European only size)
NCM0013E Columbia Agar, 50 kg (US only size)

Reference Material

NCM0013 - Columbia Agar, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0013 - Columbia Agar, Technical Specifications Sheet [English]

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