Product number: NCM0013
Columbia Agar is used with or without blood for the isolation and cultivation of a wide variety of fastidious microorganisms. Conforms to Harmonized USP/EP/JP Requirements. Columbia Agar is not intended for use in the diagnosis of disease or other conditions in humans.
A medium recommended by the Harmonized USP/EP/JP for isolation and identification of Clostridia from non-sterile products. Conforms to Harmonized USP/EP/JP performance specification. Originally described as a general purpose nutritious agar base by Ellner et al. at Columbia University that can be enriched by the addition of sterile blood. The peptone mixture and yeast extract provide a source of nitrogen, essential vitamins and amino acids. The starch provides a carbon source and sodium chloride maintains osmotic balance. The Harmonized European Pharmacopoeia states that where necessary, gentamicin sulfate at a concentration of 20mg/L can be added after sterilization to reduce the growth of non-target organisms. According to the Harmonized European Pharmacopoeia, Reinforced Medium for Clostridia is used as a selective enrichment broth, with subculture performed onto Columbia Agar.
|Pancreatic Digest of Casein||10.0 g/L|
|Meat Peptic Digest||5.0 g/L|
|Heart Pancreatic Digest||3.0 g/L|
|Yeast Extract||5.0 g/L|
|Maize Starch||1.0 g/L|
|Sodium Chloride||5.0 g/L|
Final pH: 7.3 ± 0.2 at 25 C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
- Dissolve 41 g of the medium in one liter of purified water.
- Heat with frequent agitation and boil for one minute to completely dissolve the medium.
- Autoclave at 121°C for 15 minutes.
- Cool 45-50°C.
Product was previously known as Acumedia Product: 7734 Columbia Agar; and LabM Product: HP012 Columbia Agar (USP/EP/JP)
|Product #||Product Description|
|NCM0013A||Columbia Agar, 500 g|
|NCM0013B||Columbia Agar, 5 kg|
|NCM0013C||Columbia Agar, 10 kg|
|NCM0013D||Columbia Agar, 25 kg (European only size)|
|NCM0013E||Columbia Agar, 50 kg (US only size)|
NCM0013 - Columbia Agar, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0013 - Columbia Agar, Technical Specifications Sheet [English]
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