Product number: NCM0128

Clostridium Difficile Agar Base (Brazier’s) is used with blood, Cycloserine, and Cefoxitin for the isolation of Clostridium difficile. Clostridium Difficile is not intended for use in the diagnosis of disease or other conditions in humans.

Brazier’s CCEY agar is the formulation currently used by the Anaerobe Reference Unit for the isolation of C.difficile, resulting from work initiated by Ken Phillips and Paul Levett, and completed by Jon Brazier. Based upon the market leading anaerobe medium, Fastidious Anaerobe Agar, it incorporates additional ingredients to improve the isolation and differentiation of C.difficile from samples.

Cholic acid is present to promote spore germination following alcohol shock treatment, and p-hydroxyphenylacetic acid to enhance the production of p-cresol, a distinctive metabolite of C.difficile. Selectivity is achieved by addition of supplement X093 (cefoxitin cycloserine) and egg yolk emulsion X073 is added to help differentiate C.difficile from lecithinase positive clostridia. Finally, the addition of lysed horse blood optimizes the recognition of colony fluorescence when cultures are examined using UV light.

 

Formula Liter
Peptone Mix 23.0 g/L
Sodium Chloride 5.0 g/L
Soluble Starch 1.0 g/L
Agar 12.0 g/L
Sodium Bicarbonate 0.4 g/L
Glucose 1.0 g/L
Sodium Pyruvate 1.0 g/L
Cysteine HCl 0.5 g/L
Hemin 0.01 g/L
Vitamin K 0.001 g/L
L-Arginine 1.0 g/L
Soluble Pyrophosphate 0.25 g/L
Sodium Succinate 0.5 g/L
Cholic Acid 1.0 g/L
p-Hydroxyphenylacetic acid 1.0 g/L

Final pH: 7.0 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Preparation

  1. Suspend 48 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C and aseptically add the following: 2 vials of Cycloserine/Cefoxitin Selective Supplement X093, 40 mL of Egg Yolk Emulsion X075 and 10 mL lysed horse blood.
  5. Mix well and dispense.

Product was previously known as Acumedia Product: 7385 Clostridium difficile Agar; and LabM Product: LAB160 Brazier's CCEY Agar

Order Details

Product # Product Description
NCM0128A Clostridium Difficile Agar Base (Brazier’s), 500 g
NCM0128B Clostridium Difficile Agar Base (Brazier’s), 5 kg
NCM0128C Clostridium Difficile Agar Base (Brazier’s), 10 kg
NCM0128D Clostridium Difficile Agar Base (Brazier’s), 25 kg (European only size)
NCM0128E Clostridium Difficile Agar Base (Brazier’s), 50 kg (US only size)

Reference Material

NCM0128 - Clostridium difficile Agar Base, Brazier’s, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0128 - Clostridium difficile Agar Base, Brazier’s, Technical Specifications Sheet [English]

For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.

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