Product number: NCM0220

CLED Medium (Bevis Modification) is for the cultivation of coliforms, staphylococci and streptococci, and is not intended for use in the diagnosis of disease or other conditions in humans.

Bevis modified Mackey and Sandys original medium by introducing a double indicator to improve the differentiation of lactose and non-lactose fermenting coliforms, staphylococci and streptococci. The swarming of Proteus spp. is inhibited. Neogen’s NCM0220 CLED Medium will grow many of the more demanding streptococci of Lancefield groups A, B, C, G and F. This medium may not grow Pasteurella spp. or halophilic organisms.


Formula Liter
Balanced Peptone 4.0 g/L
Beef Extract 3.0 g/L
Tryptone 4.0 g/L
Lactose 10.0 g/L
L-Cystine 0.128 g/L
Bromothymol Blue Indicator 0.02 g/L
Andrade’s Indicator 0.08 g/L
Agar 15.0 g/L

Final pH: 7.5 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 36 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C.

Product was previously known as LabM Product: LAB006 CLED Medium (Bevis)

Order Details

Product # Product Description
NCM0220A CLED Medium (Bevis Modification), 500 g
NCM0220B CLED Medium (Bevis Modification), 5 kg
NCM0220C CLED Medium (Bevis Modification), 10 kg
NCM0220D CLED Medium (Bevis Modification), 25 kg (European only size)
NCM0220E CLED Medium (Bevis Modification), 50 kg (US only size)

Reference Material

NCM0220 - CLED Medium (Bevis Modification), Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0220 - CLED Medium (Bevis Modification), Technical Specifications Sheet [English]

For additional information or questions about product SDS documents, please contact Neogen at

Please check with your local representative for availability of these products within your region.