Product number: NCM2017

Cystine Lactose Electrolyte Deficient (CLED) Agar is used for the differentiation and enumeration of microorganisms in urine.

A medium for urine culture where the absence of electrolytes inhibits the swarming of Proteus spp. Cystine is added for the benefit of those organisms which have a specific cystine requirement. Differentiation of lactose and non-lactose fermenters is achieved using bromothymol blue as pH indicator. This medium supports the growth of Streptococcus pyogenes and most other fastidious organisms that do not require blood.

 

Formula Liter
Balanced Peptone 4.0 g/L
Beef Extract 3.0 g/L
Tryptone 4.0 g/L
Lactose 10.0 g/L
L-Cystine 0.128 g/L
Bromothymol Blue 0.02 g/L
Agar 15.0 g/L

Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Preparation

  1. Suspend 36 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C.

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Order Details

Product # Product Description
NCM2017A Cystine Lactose Electrolyte Deficient (CLED) Agar CE, 500 g
NCM2017B Cystine Lactose Electrolyte Deficient (CLED) Agar CE, 5 kg
NCM2017C Cystine Lactose Electrolyte Deficient (CLED) Agar CE, 10 kg
NCM2017D Cystine Lactose Electrolyte Deficient (CLED) Agar CE, 25 kg (European only size)

Reference Material

NCM2017 - Cystine Lactose Electrolyte Deficient (CLED) Agar CE, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Hungarian] [Italian] [Spanish, Mexico] [Swedish]
NCM2017 - Cystine Lactose Electrolyte Deficient (CLED) Agar CE, Technical Specifications Sheet [English]

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