Product number: NCM0109

Cetrimide Agar is used in the isolation and identification of Pseudomonas aeruginosa and is not intended for use in the diagnosis of disease or other conditions in humans. Conforms to Harmonized USP/EP/JP Requirements.

A medium recommended by the Harmonized USP/EP/JP for the isolation and identification of Pseudomonas aeruginosa, in non- sterile pharmaceutical samples. Conforms to USP/EP/JP performance specification. Gelatin is a source of nitrogen while glycerol acts as a carbon source. Cetrimide is a quaternary ammonium compound that inhibits the growth of a wide range of Gram-positive and some Gram-negative micro-organisms. Magnesium chloride and Dipotassium sulphate improve the production of pyoverdin and pyocyanin pigments that combine to give Pseudomonas aeruginosa characteristic green colonies. According to the Harmonized USP/EP/JP, subculture is carried out onto the medium after enrichment in Casein Soya Bean Digest Broth.


Formula Liter
Pancreatic Digest of Gelatin 20.0 g/L
Magnesium Chloride 1.4 g/L
Dipotassium Sulfate 10.0 g/L
Cetrimide 0.3 g/L
Agar 13.6 g/L

Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 45.3 grams of the medium in 1 liter of purified water.
  2. Add 10mL of Glycerol.
  3. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  4. Autoclave at 121°C for 15 minutes.
  5. Cool to 45-50°C.

Product was previously known as Acumedia Product: 7688 Cetrimide Agar (Agar Medium N); and LabM Product: HP010 Cetrimide Agar (USP/EP/JP)

Order Details

Product # Product Description
NCM0109A Cetrimide Agar, 500 g
NCM0109B Cetrimide Agar, 5 kg
NCM0109C Cetrimide Agar, 10 kg
NCM0109D Cetrimide Agar, 25 kg (European only size)
NCM0109E Cetrimide Agar, 50 kg (US only size)

Reference Material

NCM0109 - Cetrimide Agar, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0109 - Cetrimide Agar, Technical Specifications Sheet [English]

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