Product number: NCM0094

Campylobacter Enrichment Broth (Bolton Broth) is used with antimicrobics for the selective enrichment of Campylobacter spp. Campylobacter Enrichment Broth (Bolton Broth) is not intended for use in the diagnosis of disease or other conditions in humans.

Campylobacter spp. are microaerophilic, very small, curved, thin, Gram-negative rods. Microaerophilic organisms have a tendency to be more sensitive to toxic forms of oxygen. Campylobacter Enrichment Broth (Bolton), along with nutritional ingredients, contains compounds which enhance the aerotolerance of microaerophilic bacteria by suppressing the toxic form of oxygen. Campylobacter Enrichment Broth (Bolton) is recommended in food testing. Blood-Free Campylobacter Enrichment Broth, Bolton Broth (2X Concentration) is described by the USDA.

Enzymatic Digest of Animal Tissue, Lactalbumin Hydrolysate, and Yeast Extract provide nitrogen, carbon, amino acids, and vitamins in Campylobacter Enrichment Broth. Hemin and Lysed Horse Blood provide essential growth factors. Sodium Chloride maintains the osmotic balance of the medium. Sodium Pyruvate, Sodium Metabisulfite, and Sodium Carbonate increase the aerotolerance of Campylobacter spp. by acting as oxygen scavengers. The addition of cefoperazone, cycloheximide, trimethoprim, and vancomycin are selective agents for heavily contaminated samples.

 

Formula Liter
Enzymatic Digest of Animal Tissue 10.0 g/L
Lactalbumin Hydrolysate 5.0 g/L
Yeast Extract 5.0 g/L
Sodium Chloride 5.0 g/L
Hemin 0.01 g/L
Sodium Pyruvate 0.5 g/L
α-Ketoglutaric Acid 1.0 g/L
Sodium Metabisulfite 0.5 g/L
Sodium Carbonate 0.6 g/L

Final pH: 7.4 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications. If following USDA-MLG method, use Campylobacter Supplement (Bolton), 5 mL (7998).
Alternatively, use CVTN Supplement, 5 mL (X132)

Preparation

  1. Dissolve 27.6 g of the medium in one liter of purified water.
  2. Heat with frequent agitation to completely dissolve the medium, if necessary.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45 - 50°C and aseptically add 50 mL of lysed horse blood and 2 vials of Campylobacter Supplement - Bolton (7998) or CVTN Supplement (X132).
  5. Note: Blood-Free Campylobacter Enrichment Broth, Bolton’s (2X Concentration) is described by the USDA.

Product was previously known as Acumedia Product: 7526 Campylobacter Enrichment Broth (Bolton’s); and LabM Product: LAB135 Campylobacter Enrichment Broth

Order Details

Product # Product Description
NCM0094A Campylobacter Enrichment Broth (Bolton Broth), 500 g
NCM0094B Campylobacter Enrichment Broth (Bolton Broth), 5 kg
NCM0094C Campylobacter Enrichment Broth (Bolton Broth), 10 kg
NCM0094D Campylobacter Enrichment Broth (Bolton Broth), 25 kg (European only size)
NCM0094E Campylobacter Enrichment Broth (Bolton Broth), 50 kg (US only size)

Reference Material

NCM0094 - Campylobacter Enrichment Broth (Bolton Broth), Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0094 - Campylobacter Enrichment Broth (Bolton Broth), Technical Specifications Sheet [English]

For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.

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