Product number: NCM0048

Brilliant Green Bile Broth 2% is used for the detection of coliform bacteria in water, food, and dairy products in a laboratory setting. Brilliant Green Bile Broth 2% is not intended for use in the diagnosis of disease or other conditions in humans.

The coliform group of bacteria includes aerobic and facultative anaerobic, Gram-negative, non-sporeforming bacilli that ferment lactose and form acid and gas at 35°C within 48 hours. Members of the Enterobacteriacae comprise the majority of this group, but organisms such as Aeromonas spp. may also be included. Procedures to detect and confirm coliforms are used in testing water, foods, dairy products and other materials. Brilliant Green Bile Broth 2% is used to confirm a positive presumptive test result.

Brilliant Green Bile Broth 2% is also referred to as Brilliant Green Bile Broth, Brilliant Green Lactose Broth, Brilliant Green Lactose Bile Broth and Brilliant Green Lactose Bile Broth, 2%.

 

Formula Liter
Enzymatic Digest of Gelatin 10.0 g/L
Lactose 10.0 g/L
Ox Bile 20.0 g/L
Brilliant Green 0.0133 g/L

Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Preparation

  1. Dissolve 40 g of the medium in one liter of purified water until evenly dispersed.
  2. Mix thoroughly.
  3. Distribute into bottles or tubes with inverted Durham tubes.
  4. Autoclave at 115°C for 15 minutes. To avoid entrapment of bubbles in the fermentation tubes, allow the autoclave to cool at least to 75°C before opening.

Product was previously known as Acumedia Product: 7119 Brilliant Green Bile Broth 2%; and LabM Product: LAB051 Brilliant Green Bile 2% Broth

Order Details

Product # Product Description
NCM0048A Brilliant Green Bile 2% Broth, 500 g
NCM0048B Brilliant Green Bile 2% Broth, 5 kg
NCM0048C Brilliant Green Bile 2% Broth, 10 kg
NCM0048D Brilliant Green Bile 2% Broth, 25 kg (European only size)
NCM0048E Brilliant Green Bile 2% Broth, 50 kg (US only size)

Reference Material

NCM0048 - Brilliant Green Bile 2% Broth, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0048 - Brilliant Green Bile 2% Broth, Technical Specifications Sheet [English]

For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.

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