Product number: NCM0016

Brain Heart Infusion Broth is used for the cultivation of a wide variety of fastidious organisms and is not intended for use in the diagnosis of disease or other conditions in humans.

A rich isotonic infusion medium with tryptose (a mixture of meat and milk peptones) providing a wide range of substrates. A low concentration of glucose is used to stimulate early growth. The medium is lightly buffered to prevent the early death of some species due to acid production. Organisms which produce significant amounts of acid may well overwhelm the buffering system and auto-sterilize. The medium is suitable for use as a blood culture medium or as an enrichment broth for fastidious organisms.


Formula Liter
Brain Heart Infusion Solids (Porcine) 17.5 g/L*
Tryptose 10.0 g/L
Glucose 2.0 g/L
Sodium Chloride 5.0 g/L
Disodium Hydrogen Phosphate 2.5 g/L

*Equivalent to 12.5 g dehydrated brain infusion and 5.0 g of heart infusion
Final pH: 7.4 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Dissolve 37 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation to completely dissolve the medium, if necessary.
  3. Autoclave at 121°C for 15 minutes.

Product was previously known as Acumedia Product: 7116 Brain-Heart Infusion Broth; and LabM Product: LAB049 Brain Heart Infusion Broth

Order Details

Product # Product Description
NCM0016A Brain Heart Infusion Broth, 500 g
NCM0016B Brain Heart Infusion Broth, 5 kg
NCM0016C Brain Heart Infusion Broth, 10 kg
NCM0016D Brain Heart Infusion Broth, 25 kg (European only size)
NCM0016E Brain Heart Infusion Broth, 50 kg (US only size)

Reference Material

NCM0016 - Brain Heart Infusion Broth, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0016 - Brain Heart Infusion Broth, Technical Specifications Sheet [English]

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