Product number: NCM0080

Brain Heart Infusion Agar is used for the cultivation of a wide variety of fastidious organisms and is not intended for use in the diagnosis of disease or other conditions in humans.

A general purpose nutritious agar base. This medium was first used for the isolation of dental pathogens. With the addition of 7% defibrinated blood the medium will support the growth of a wide range of fastidious organisms, the phosphate buffer will help neutralize the acids produced from the utilization of glucose and thus maintain viability. The medium is not recommended for the determination of hemolytic reactions because of the glucose content.


Formula Liter
Brain-Heart Infusion Solids 17.5 g/L
Tryptose 10.0 g/L
Glucose 2.0 g/L
Sodium Chloride 5.0 g/L
Disodium Phosphate 2.5 g/L
Agar 12.0 g/L

Final pH: 7.4 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 49 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Product was previously known as Acumedia Product: 7115 Brain Heart Infusion Agar; and LabM Product: LAB048 Brain Heart Infusion Agar

Order Details

Product # Product Description
NCM0080A Brain Heart Infusion Agar, 500 g
NCM0080B Brain Heart Infusion Agar, 5 kg
NCM0080C Brain Heart Infusion Agar, 10 kg
NCM0080D Brain Heart Infusion Agar, 25 kg (European only size)
NCM0080E Brain Heart Infusion Agar, 50 kg (US only size)

Reference Material

NCM0080 - Brain Heart Infusion Agar, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0080 - Brain Heart Infusion Agar, Technical Specifications Sheet [English]

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