Product number: NCM2013

Blood Agar Base No. 2 is used with blood for the isolation and cultivation of a wide variety of fastidious microorganisms.

A very rich agar base which, with the addition of blood, is capable of growing fastidious organisms. The medium gives colonial appearances, hemolysis patterns and pigment production of indicative value. When the blood is 'chocolated' the medium gives good recovery of Haemophilus spp. The medium can be made selective for various groups by the addition of appropriate antibiotic mixtures eg:

  • Streptococcus spp. Colistin/Oxolinic acid (X013)
  • Gardnerella spp. Colistin/Oxolinic acid (X011)
  • C. perfringens Neomycin (X015) (X016)
  • Staphylococcus/Streptococcus spp. Colistin/Naladixic acid (X012)


Formula Liter
Tryptose 15.0 g/L
Soy Peptone 2.5 g/L
Yeast Extract 5.0 g/L
Sodium Chloride 5.0 g/L
Agar 12.0 g/L

pH: 7.4 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 39.5 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C.
  5. Aseptically add 5-7% sterile, defibrinated horse or sheep blood.
  6. Mix well before pouring.

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Order Details

Product # Product Description
NCM2013A Blood Agar Base No. 2 CE, 500 g
NCM2013B Blood Agar Base No. 2 CE, 5 kg
NCM2013C Blood Agar Base No. 2 CE, 10 kg
NCM2013D Blood Agar Base No. 2 CE, 25 kg (European only size)

Reference Material

LAB015 - Blood Agar Base No. 2 CE, Safety Data Sheet [Danish] [Danish] [Dutch] [Dutch] [English] [English] [EU English] [EU English] [Finnish] [Finnish] [French] [French] [German] [German] [Hungarian] [Italian] [Italian] [Spanish, Mexico] [Spanish, Mexico] [Swedish] [Swedish]
NCM2013 - Blood Agar Base No. 2 CE, Technical Specifications Sheet [English]

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