Product number: NCM0175

Alkaline Saline Peptone Water is a medium for the enrichment of Vibrio spp. from food and water samples according to ISO 21872-1:2017 and ISO 21872-2:2007, and is not intended for use in the diagnosis of disease or other conditions in humans.

Originally described by Shread, Donovan & Lee as an enrichment broth for Aeromonas spp. and identified by Cruickshank as an effective medium for the enrichment of Vibrio spp., Alkaline Saline Peptone Water uses elevated pH and salt levels to provide a favorable environment for the enrichment of Vibrio spp.

Peptone provides essential vitamins, minerals, amino acids & nitrogen for growth requirements. Sodium chloride provides essential electrolytes for maintenance of the osmotic balance.

Sub-culture onto Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar (NCM0052) will be required.


Formula Liter
Peptone 20.0 g/L
Sodium Chloride 20.0 g/L

Final pH: 8.6 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Dissolve 40 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation to completely dissolve the medium, if necessary.
  3. Autoclave at 121°C for 15 minutes.

Product was previously known as LabM Product: LAB224 ASPW - Alkaline Saline Peptone Water (ISO)

Order Details

Product # Product Description
NCM0175A Alkaline Saline Peptone Water (ASPW) (ISO), 500 g
NCM0175B Alkaline Saline Peptone Water (ASPW) (ISO), 5 kg
NCM0175C Alkaline Saline Peptone Water (ASPW) (ISO), 10 kg
NCM0175D Alkaline Saline Peptone Water (ASPW) (ISO), 25 kg (European only size)
NCM0175E Alkaline Saline Peptone Water (ASPW) (ISO), 50 kg (US only size)

Reference Material

NCM0175 - Alkaline Saline Peptone Water (ASPW) (ISO), Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0175 - Alkaline Saline Peptone Water (ASPW) (ISO), Technical Specifications Sheet [English]

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