Product number: 7736

Modified Buffered Peptone Water with Pyruvate (mBPWp) is used for the isolation of Enterohemorrhagic E. coli (EHEC) in a laboratory setting. Modified Buffered Peptone Water with Pyruvate (mBPWp) is not intended for use in the diagnosis of disease or other conditions in humans.

Enterohemorrhagic E. coli (EHEC) are recognized as the primary cause of hemorrhagic colitis (HC) or bloody diarrhea. This infection can also lead to hemolytic uremic syndrome (HUS). Enterohemorrhagic infections are usually food or water borne and have been implicated in undercooked ground beef, raw milk, lunchmeat, water, unpasteurized apple cider, sprouts and vegetables.

Modified Buffered Peptone Water with Pyruvate is recommended in the sample preparation for the screening method following FDA/BAM Enterohemorrhagic E. coli (EHEC) Protocol. After the sample is incubated for five hours in Modified Buffered Peptone Water with Pyruvate, several antimicrobial agents are added, followed by an additional incubation period. These antimicrobic supplements effectively suppress normal flora, while allowing the growth of viable O157:H7 cells (including STEC), and is capable of detecting < 1 cfu/g in foods. Improved recovery of pathogenic E. coli from difficult commodities was achieved with Modified Buffered Peptone Water with Pyruvate enrichment.

 

Formula Liter
Enzymatic Digest of Casein 10 g
Lactose 10 g
Yeast Extract 6 g
Acid Digest of Casein 5 g
Sodium Chloride 5 g
Sodium Phosphate, dibasic 3.6 g
Potassium Phosphate, monobasic 1.5 g
Sodium Pyruvate 1 g

Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Dissolve 42.1 g of the medium in one liter of purified water.
  2. Heat with frequent agitation to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7736A Modified Buffered Peptone Water with Pyruvate, 500 g
7736T Modified Buffered Peptone Water with Pyruvate, 5 kg
7736C Modified Buffered Peptone Water with Pyruvate, 10 kg

Reference Material

7736 - Buffered Peptone Water, Modified w/Pyruvate, Product Information Sheet [English]
7736 - Buffered Peptone Water, Modified w/Pyruvate, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]

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