Product number: 7236
MacConkey Agar without Crystal Violet is used for the isolation and differentiation of Gram-negative enteric bacilli in a laboratory setting. MacConkey Agar without Crystal Violet is not intended for use in the diagnosis of disease or other conditions in humans.
MacConkey Agar is based on the bile salt-neutral red-lactose agar of MacConkey. The original MacConkey medium was used to differentiate strains of Salmonella typhosa from members of the coliform group. Formula modifications improved growth of Shigella and Salmonella strains. These modifications include the addition of 0.5% sodium chloride, decreased agar content, altered bile salts, and neutral red concentrations. The formula changes improved differential reactions between enteric pathogens and coliforms.
MacConkey Agar without Crystal Violet is a differential medium and less selective than MacConkey Agar. The lack of Crystal Violet permits growth of enterococci, staphylococci, and Mycobacterium spp.
|Enzymatic Digest of Gelatin||17 g|
|Enzymatic Digest of Casein||1.5 g|
|Enzymatic Digest of Animal Tissue||1.5 g|
|Bile Salts Mixture||5 g|
|Sodium Chloride||5 g|
|Neutral Red||0.05 g|
Final pH: 7.4 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
- Suspend 52 g of the medium in one liter of purified water.
- Heat with frequent agitation and boil for one minute to completely dissolve the medium.
- Autoclave at 121°C for 15 minutes.
|Product #||Product Description|
|7236A||MacConkey Agar without Crystal Violet, 500 g|
|7236T||MacConkey Agar without Crystal Violet, 5 kg|
|7236C||MacConkey Agar without Crystal Violet, 10 kg|