Product no: 7397

m-FC Agar is used with rosolic acid for the detection and enumeration of fecal coliforms by membrane filtration in a laboratory setting. m-FC Agar is not intended for use in the diagnosis of disease or other conditions in humans.

Geldreich et al. formulated a medium to enumerate fecal coliforms (FC) using the membrane filter (m) technique without prior enrichment.

Many standard method membrane filtration procedures recommend m-FC media for testing water. The American Public Health Association (APHA) specified m-FC media and incubation at 44.5 ± 0.5°C in the fecal coliform procedure and other tests. The Association of Official Analytical Chemists (AOAC) specifies m-FC Agar for detecting total coliforms and fecal coliforms in foods. The US Environmental Protection Agency specified using m-FC media in fecal coliform methods for testing water by the direct MF method or the delayed-incubation MF methods.

 

Formula Liter
Enzymatic Digest of Casein 10 g
Enzymatic Digest of Animal Tissue 5 g
Yeast Extract 3 g
Sodium Chloride 5 g
Lactose 12.5 g
Bile Salts 1.5 g
Aniline Blue 0.1 g
Agar 15 g

Final pH: 7.4 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Supplement

1% Rosolic Acid, 10 mL

Directions

m-FC Agar

  1. Suspend 52 g of the medium in 1 L of purified water containing 10 mL of 1% Rosolic Acid in 0.2 N NaOH.
  2. If necessary, adjust pH to 7.4 with 1N HCl.
  3. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  4. Cool to 45 – 50°C and pour plates.
  5. DO NOT AUTOCLAVE.

Rosolic Acid

  1. Dissolve 1 g in 100 mL of 0.2 N NaOH to prepare a 1% solution.

Order Details

Product # Product Description
7397A m-FC Agar, 500 g
7397T m-FC Agar, 5 kg
7397C m-FC Agar, 10 kg

Reference Material

7397 - m-FC Agar, Product Information Sheet [English] [Portuguese]
7397 - m-FC Agar, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Hungarian] [Italian] [Spanish, Mexico] [Swedish]

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