Product no: 7397
m-FC Agar is used with rosolic acid for the detection and enumeration of fecal coliforms by membrane filtration in a laboratory setting. m-FC Agar is not intended for use in the diagnosis of disease or other conditions in humans.
Geldreich et al. formulated a medium to enumerate fecal coliforms (FC) using the membrane filter (m) technique without prior enrichment.
Many standard method membrane filtration procedures recommend m-FC media for testing water. The American Public Health Association (APHA) specified m-FC media and incubation at 44.5 ± 0.5°C in the fecal coliform procedure and other tests. The Association of Official Analytical Chemists (AOAC) specifies m-FC Agar for detecting total coliforms and fecal coliforms in foods. The US Environmental Protection Agency specified using m-FC media in fecal coliform methods for testing water by the direct MF method or the delayed-incubation MF methods.
|Enzymatic Digest of Casein||10 g|
|Enzymatic Digest of Animal Tissue||5 g|
|Yeast Extract||3 g|
|Sodium Chloride||5 g|
|Bile Salts||1.5 g|
|Aniline Blue||0.1 g|
Final pH: 7.4 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
1% Rosolic Acid, 10 mL
- Suspend 52 g of the medium in 1 L of purified water containing 10 mL of 1% Rosolic Acid in 0.2 N NaOH.
- If necessary, adjust pH to 7.4 with 1N HCl.
- Heat with frequent agitation and boil for one minute to completely dissolve the medium.
- Cool to 45 – 50°C and pour plates.
- DO NOT AUTOCLAVE.
- Dissolve 1 g in 100 mL of 0.2 N NaOH to prepare a 1% solution.
|Product #||Product Description|
|7397A||m-FC Agar, 500 g|
|7397T||m-FC Agar, 5 kg|
|7397C||m-FC Agar, 10 kg|