Product number: 7296

M-Broth is used for the cultivation of Salmonella spp. in a laboratory setting. M-Broth is not intended for use in the diagnosis of disease or other conditions in humans.

M-Broth is prepared according to the formula of Sperber and Diebel, and contains the nutrients necessary for good growth and flagella development of Salmonella. M-Broth is used for cultivating Salmonella in foods and feeds by the accelerated enrichment serology (ES) procedure. M-Broth conforms to standard procedures recommended in food testing and monoclonal and polyclonal enzyme immunoassays (EIA).

 

Formula Liter
Enzymatic Digest of Casein 12.5 g
Yeast Extract 5 g
D-Mannose 2 g
Sodium Citrate 5 g
Sodium Chloride 5 g
Potassium Phosphate 5 g
Manganese Chloride 0.14 g
Magnesium Sulfate 0.8 g
Ferrous Sulfate 0.04 g
Polysorbate 80 0.75 g

Final pH: 7.0 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Dissolve 36.2 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7296A M-Broth, 500 g
7296T M-Broth, 5 kg
7296C M-Broth, 10 kg

Reference Material

7296 - M-Broth, Product Information Sheet [English] [Portuguese]
7296 - M-Broth, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Hungarian] [Italian] [Spanish, Mexico] [Swedish]

For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.

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