LMG Agar (Lactose Monensin Glucuronate Agar) is used in the selective and differential isolation of coliform bacteria, using the ISO-GRID® and /or NEO-GRID® Membrane Filtration System. LMG Agar is not intended for use in the diagnosis of disease or other conditions in humans.
Lactose Monensin Glucuronate Agar is based on the coliform enumeration medium, m-FC Agar. The original m-FC formula was designed for fecal coliform enumeration from water samples using membrane filtration. This medium was modified for enumeration of total coliforms by eliminating rosolic acid supplement and reducing the incubation temperature. A formula change to modified m-FC Agar included the addition of Monensin to improve recovery of injured coliforms.
LMG Agar is recommended for the detection and enumeration of total confirmed coliform bacteria from all foods using the ISO-GRID and /or NEO-GRID Membrane Filtration System method.
|Enzymatic Digest of Casein||10 g|
|Enzymatic Digest of Animal Tissue||5 g|
|Yeast Extract||3 g|
|Sodium Deoxycholate||0.15 g|
|Aniline Blue||0.1 g|
|Glucuronic Acid, Sodium Salt||0.5 g|
Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
- Suspend 46.2 g of the medium in one liter of purified water.
- Heat with frequent agitation and boil for one minute to completely dissolve the medium.
- DO NOT AUTOCLAVE.
- Cool to 45 – 50° C.
- Determine pH and adjust if necessary to 7.2 ± 0.2.
|Product #||Product Description|
|6902A||LMG Agar, 500 g|