Product number: 7173

Littman Agar is used for the isolation and cultivation of fungi in a laboratory setting. Littman Agar is not intended for use in the diagnosis of disease or other conditions in humans.

In 1947, Littman Agar was described as suitable for growth of pathogenic fungi at a neutral pH. Littman Agar is a selective medium for the primary isolation of fungi. Littman demonstrated this medium as valuable for culturing dermatophytes. Molds and yeasts form nonspreading, discrete colonies easily isolated in pure culture. Littman Agar can be used for single cell isolation of fungi and plate counts of viable saprophytic fungi in foodstuffs and air.

Littman compared Littman Agar with Sabouraud Dextrose Agar using a large variety of pathogenic and saprophytic fungi. Four times as many pathogenic dermatophytes grew on Littman Agar as compared with Sabouraud Dextrose Agar.

 

Formula Liter
Enzymatic Digest of Gelatin 10 g
Oxgall 15 g
Dextrose 10 g
Crystal Violet 0.01 g
Agar 16 g

Final pH: 7.0 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Supplement

Streptomycin, 30 mcg

Directions

  1. Suspend 51 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes. Cool to 45 – 50°C and add 30 mcg of Streptomycin per mL of medium.

Order Details

Product # Product Description
7173A Littman Agar, 500 g
7173B Littman Agar, 2 kg
7173T Littman Agar, 5 kg

Reference Material

7173 - Littman Agar, Product Information Sheet [English]

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