Product number: 7496
Letheen Broth Base, Modified is used with Polysorbate 80 for the recovery of microorganisms from cosmetics in a laboratory setting. Letheen Broth Base, Modified is not intended for use in the diagnosis of disease or other conditions in humans.
In 1948, Weber and Black described the value of a highly nutritional solid medium containing neutralizing agents for quaternary ammonium compounds in sanitizers. The addition of Lecithin and Polysorbate 80 to Tryptone Glucose Extract (TGE) Agar resulted in a medium that effectively neutralizes quaternary ammonium compounds in testing of germicidal activity. Total neutralization of disinfectants is critical. Disinfectant residues can result in a false negative (no-growth) test.
Letheen Broth Base, Modified is based on the formula described in FDA Bacteriological Analytical Manual, and a modification of Letheen Broth Base. Letheen Broth Base, Modified is recommended by the FDA for use in the microbiological testing of cosmetics.
|Letheen Broth Base||20.7 g|
|Enzymatic Digest of Casein||5 g|
|Enzymatic Digest of Animal Tissue||10 g|
|Yeast Extract||2 g|
|Sodium Bisulfite||0.1 g|
Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
Supplement / Liter
Tween 80 (Polysorbate 80), 5 g
- Dissolve 37.8 g of the medium and 5 g of Tween 80 (Polysorbate 80) (#7992) in one liter of purified water.
- Heat with frequent agitation to completely dissolve the medium.
- Autoclave at 121°C for 15 minutes.
|Product #||Product Description|
|7496A||Letheen Broth Base, Modified, 500 g|
|7496T||Letheen Broth Base, Modified, 5 kg|
|7496C||Letheen Broth Base, Modified, 10 kg|
|7992||Tween 80 (Polysorbate 80), 100 mL|