Product number: 7279

LB Broth, Miller is used in molecular genetic studies in a laboratory setting. LB Broth, Miller is not intended for use in the diagnosis of disease or other conditions in humans.

LB Broth, Miller is based on the formula described by Miller. This medium is used for the growth and maintenance of Escherichia coli strains used in molecular microbiology procedures. LB Broth, Miller is a nutritionally rich medium designed for growth of pure cultures of recombinant strains. E. coli is grown to late log phase in LB Medium. Some plasmid vectors replicate to high copy numbers without selective amplification. Some vectors do not replicate so freely, and need to be selectively amplified. Chloramphenicol can be added to inhibit host synthesis and prevent replication of the bacterial chromosome.

LB Broth, Miller contains 10 g/L of sodium chloride. The medium may be aseptically supplemented with glucose.

 

Formula Liter
Enzymatic Digest of Casein 10 g
Yeast Extract 5 g
Sodium Chloride 10 g

Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Dissolve 25 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7279A LB Broth, Miller, 500 g
7279T LB Broth, Miller, 5 kg
7279C LB Broth, Miller, 10 kg

Reference Material

7279 - LB Broth, Miller, Product Information Sheet [English] [Portuguese]
7279 - LB Broth, Miller, Safety Data Sheet [English] [Portuguese]

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