Product number: 7140

Kligler Iron Agar is used for the differentiation of microorganisms on the basis of dextrose and lactose fermentation and hydrogen sulfide production in a laboratory setting. Kligler Iron Agar is not intended for use in the diagnosis of disease or other conditions in humans.

Kligler Iron Agar is a modification of Kligler's original formula, recommended to identify pure cultures of colonies picked from primary plating media. The original medium was a soft nutrient agar containing dextrose, Andrade indicator and lead acetate. Russell devised a medium containing glucose, lactose, and an indicator for the differentiation of lactose-fermenting and nonlactose-fermenting Gram-negative bacilli. Kligler found lead acetate could detect hydrogen sulfide when combined with Russell double sugar medium for the differentiation of typhoid, partyphoid, and dysentery groups. Bailey and Lacy simplified the formula by using phenol red as the pH indicator instead of Andrade indicator. A similar medium containing sucrose, tryptone, ferrous sulfate, and thiosulfate was developed by Sulkins and Willet.

Kligler Iron Agar is recommended for differentiation of enteric Gram-negative bacilli from food samples.


Formula Liter
Enzymatic Digest of Casein 10 g
Enzymatic Digest of Animal Tissue 10 g
Lactose 10 g
Dextrose 1 g
Ferric Ammonium Citrate 0.5 g
Sodium Chloride 5 g
Sodium Thiosulfate 0.5 g
Phenol Red 0.025 g
Agar 15 g

Final pH: 7.4 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 52 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Distribute into test tubes and autoclave for 15 minutes at 121°C.
  4. After autoclaving, allow medium to solidify in a slanted position.

Order Details

Product # Product Description
7140A Kligler Iron Agar, 500 g

Reference Material

7140 - Kligler Iron Agar, Product Information Sheet [English] [Portuguese]
7140 - Kligler Iron Agar, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]

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