NEO-GRID® disposable units and ISO-GRID® membranes are filtration systems used on food, beverage and water samples for the detection, confirmation and enumeration of microorganisms including yeast and mold, total plate counts, coliforms, generic E. coli, E. coli O157:H7, Salmonella, Listeria, Listeria monocytogenes, Staphylococcus and more.
ISO-GRID/NEO-GRID tests are based on the concept of hydrophobic grid membrane filtration. The filter membrane is embossed with hydrophobic ink to produce a grid containing 1600 individual squares. The ink’s hydrophobic nature contains the growth of an organism isolated during the filtration process within the square of capture.
Samples are first diluted in a sterile buffer. The ISO-GRID /NEO-GRID filtration unit then is placed onto a manifold connected to a vacuum source, and a sterile filter membrane is placed in the ISO-GRID filtration unit (NEO-GRID already contains a sterile filter membrane). Using the vacuum source, a portion of the diluted sample is filtered through the filter membrane.
The membrane is transferred to an agar plate prepared with a medium formulated to promote/differentiate the growth of the target organism. Following incubation, the plate’s squares containing presumptively positive colonies are counted to yield a most probable number (MPN) result.
ISO-GRID/NEO-GRID Method Approvals
Yeast and Mold Count AOAC No. 995.21
Total Bacterial Count AOAC No. 986.32
Total Coliform/E. coli Count AOAC No. 990.11
Salmonella Detection AOAC No. 991.12
E. coli O157:H7 Count AOAC No. 997.11
Canadian Compendium of Analytical Methods-Health Canada
Enumeration of coliforms in foods by the hydrophobic grid membrane filter method. MFHPB-17
Enumeration of E. coli in foods by the hydrophobic grid membrane filter method. MFHPB-26
Enumeration of fecal coliforms in foods by the hydrophobic grid membrane filter method. MFLP-55
Determination of aerobic colony count in foods by the hydrophobic grid membrane filter method. MFLP-56
Screening bottled water for the presence of indicator and pathogenic bacteria. MFLP-60
Canadian Standard Methods for the Examination of Water and Wastewater
Standard total coliform membrane filter procedure 9222B
Fecal coliform membrane filter procedure 9222D
|Product #||Product Description|
|6802||ISO-GRID Membrane Filters, standard 1600-grid with a pore size of 0.45 µm (100 per box)|
|6848||NEO-GRID Test System – Contains 48 individually packaged membrane filtration systems|
|6866||NEO-GRID-36 Test System – Contains 48 individually packaged 36-grid disposable membrane filtration systems with a pore size of 0.45 µm|
For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.