Product number: 7520
HC Agar Base is used with Tween 80 (Polysorbate 80) for the enumeration of molds in cosmetics in a laboratory setting. HC Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.
Methods for isolating molds from cosmetic products require incubation for 5 – 7 days using traditional agar media. In 1986, Mead and O'Neill described a new medium, HC Agar, for enumerating molds in cosmetic products. The formulation of HC Agar decreased incubation time to 3 days at 27.5 ± 0.5°C for molds. HC Agar Base, based on the HC Agar formula of Mead and O'Neill, is supplemented with Tween 80 (Polysorbate 80) to prepare HC Agar.
|Enzymatic Digest of Casein||2.5 g|
|Enzymatic Digest of Animal Tissue||2.5 g|
|Yeast Extract||5 g|
|Disodium Phosphate||3.5 g|
|Monopotassium Phosphate||3.4 g|
|Ammonium Chloride||1.4 g|
|Sodium Carbonate||1 g|
|Magnesium Sulfate||0.06 g|
Final pH: 7.0 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
Supplement / Liter
Tween 80, 20 mL
- Suspend 54.5 g of the medium in one liter of purified water.
- Heat with frequent agitation and boil for one minute to completely dissolve the medium.
- Add 20 mL of Tween 80 (Polysorbate 80) and mix.
- Autoclave at 121°C for 15 minutes.
|Product #||Product Description|
|7520A||HC Agar Base, 500 g|
|7520T||HC Agar Base, 5 kg|
|7520C||HC Agar Base, 10 kg|
|7992||Tween 80, 100 mL|
NCM0155 - HC Agar Base, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Hungarian] [Italian] [Spanish, Mexico] [Swedish]
7520 - HC Agar Base, Product Information Sheet [English] [Portuguese]
For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.