Product number: HAL003

Tryptone Bile Glucuronide Agar is recommended by ISO 16649 for enumeration of β-glucuronidase-positive Escherichia coli in products intended for human consumption or for the feeding of animals.

Harlequin® Tryptone Bile Glucuronide Agar was developed for the detection and enumeration of E. coli in food products. E. coli are differentiated based on their production of the β-D-glucuronidase enzyme. Bile salts are included in the formula and act as a selective agent against Gram-positive organisms. This medium conforms to the performance requirements of ISO 11133:2014.

Typical Formulation g/Liter
Tryptone 20 g
Bile Salts No. 3 1.5 g
X-glucuronide 0.075 g
Agar 15 g
Grams per liter 36.5 g

Final pH: 7.2 ± 0.2


  1. Weigh 36.5 grams of powder and disperse in 1 liter of deionized water.
  2. Allow to soak for 10 minutes, swirl to mix.
  3. Autoclave for 15 minutes at 121°C.
  4. Cool to 47°C and mix well.
  5. Dispense into Petri dishes.

Order Details

Product # Product Description
HAL003A Harlequin Tryptone Bile Glucuronide Agar – 500 g

Reference Material

HAL003 - Harlequin TBGA/TBX (Tryptone Bile Glucuronide Agar), Product Information Sheet [English]
HAL003 - Harlequin TBGA/TBX (Tryptone Bile Glucuronide Agar), Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]

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