Product number: HAL006
Harlequin® SMAC-BCIG is a specific substrate medium for the isolation of Escherichia coli O157:H7, the primary serovar associated with haemorrhagic colitis (HC) and haemolytic uraemic syndrome (HUS).
Harlequin SMAC-BCIG was developed to isolate and identify E. coli O157:H7. The medium is a modification of Sorbitol MacConkey Agar (SMAC). The addition of the chromogenic substrate BCIG (5-bromo-4-chloro-3-indoxyl-β-D-glucuronide) improves the specificity of the medium. E. coli O157:H7 is typically sorbitol negative and β-glucuronidase negative producing pale translucent colonies on this medium. The majority of other E. coli strains are β-glucuronidase positive and sorbitol positive (pink/red colonies). A small percentage of E. coli are β-glucuronidase positive and sorbitol negative and thus appear as blue/green colonies on this medium. Consequently this medium can distinguish between non-O157 sorbitol negative E. coli and the genuine toxigenic E. coli O157:H7. The medium can be made more selective by the addition of Cefixime Tellurite supplement X161 to prepare CT-SMAC. This medium can also be useful for the detection of other STEC producing E. coli in conjunction with specifically targeted IMS particles (Captivate™).
|Bile Salts No.3||1.5 g|
|Sodium Chloride||5 g|
|Neutral Red||0.03 g|
|Crystal Violet||0.001 g|
|Grams per liter||48.6 g|
Final pH: 7.1 ± 0.2
- Weigh 48.6 grams of powder and disperse in 1 liter of deionized water.
- Allow to soak for 10 minutes, swirl to mix.
- Autoclave for 15 minutes at 121°C.
- Cool to 47°C and add 2 vials of reconstituted X161 CT supplement. Swirl to mix.
- Dispense into Petri dishes.
|Code #||Product Description|
|HAL006A||Harlequin SMAC-BCIG – 500 g|
Reference MaterialHAL006 - Harlequin SMAC-BCIG, Material Safety Data Sheet [English]
HAL006 - Harlequin SMAC-BCIG, Product Information Sheet [English]