Product number: HAL006

Harlequin® SMAC-BCIG is a specific substrate medium for the isolation of Escherichia coli O157:H7, the primary serovar associated with haemorrhagic colitis (HC) and haemolytic uraemic syndrome (HUS).

Harlequin SMAC-BCIG was developed to isolate and identify E. coli O157:H7. The medium is a modification of Sorbitol MacConkey Agar (SMAC). The addition of the chromogenic substrate BCIG (5-bromo-4-chloro-3-indoxyl-β-D-glucuronide) improves the specificity of the medium. E. coli O157:H7 is typically sorbitol negative and β-glucuronidase negative producing pale translucent colonies on this medium. The majority of other E. coli strains are β-glucuronidase positive and sorbitol positive (pink/red colonies). A small percentage of E. coli are β-glucuronidase positive and sorbitol negative and thus appear as blue/green colonies on this medium. Consequently this medium can distinguish between non-O157 sorbitol negative E. coli and the genuine toxigenic E. coli O157:H7. The medium can be made more selective by the addition of Cefixime Tellurite supplement X161 to prepare CT-SMAC. This medium can also be useful for the detection of other STEC producing E. coli in conjunction with specifically targeted IMS particles (Captivate™).

Typical Formulation g/Liter
Peptone 20 g
Sorbitol 10 g
Bile Salts No.3 1.5 g
Sodium Chloride 5 g
BCIG 0.1 g
Neutral Red 0.03 g
Crystal Violet 0.001 g
Agar 12 g
Grams per liter 48.6 g

Final pH: 7.1 ± 0.2

Directions

  1. Weigh 48.6 grams of powder and disperse in 1 liter of deionized water.
  2. Allow to soak for 10 minutes, swirl to mix.
  3. Autoclave for 15 minutes at 121°C.
  4. Cool to 47°C and add 2 vials of reconstituted X161 CT supplement. Swirl to mix.
  5. Dispense into Petri dishes.

Order Details

Code # Product Description
HAL006A Harlequin SMAC-BCIG – 500 g

Reference Material

HAL006 - Harlequin SMAC-BCIG, Product Information Sheet [English]
HAL006 - Harlequin SMAC-BCIG, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]

For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.

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