Product number: HAL008

Harlequin® E. coli / Coliform Medium is used for the enumeration of Escherichia coli and coliforms in food and environmental samples.

Harlequin E. coli / Coliform Medium was developed to simultaneously enumerate E. coli and coliforms in food and environmental samples using a dual chromogenic substrate strategy. Tryptone Bile Agar is the base of the medium, which has been modified by the addition of two chromogenic substrates, one to detect the β-glucuronidase enzyme produced by E. coli and another to detect the β-galactosidase enzyme produced by coliforms.

Typical Formulation g/Liter
Tryptone 20 g
Bile Salts No.3 1.5 g
X-glucuronide 0.075 g
Magenta-β-galactoside 0.1 g
Agar 15 g
Grams per liter 36.6 g

Final pH: 7.2 ± 0.2

Directions

  1. Weigh 36.6 grams of powder and disperse in 1 liter of deionized water.
  2. Allow to soak for 10 minutes, swirl to mix.
  3. Autoclave for 15 minutes at 121°C.
  4. Cool to 47°C and mix well.
  5. Dispense into Petri dishes.

Order Details

Product # Product Description
HAL008A Harlequin E. coli / Coliform Medium – 500 g

Reference Material

HAL008 - Harlequin E. coli/Coliform Medium, Product Information Sheet [English]
HAL008 - Harlequin E. coli/Coliform Medium, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Hungarian] [Italian] [Spanish, Mexico] [Swedish]

For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.

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