Product number: 7103

Eosin Methylene Blue Agar, Levine is used for the isolation and differentiation of Gram-negative enteric bacilli in a laboratory setting. Eosin Methylene Blue Agar, Levine is not intended for use in the diagnosis of disease or other conditions in humans.

Eosin Methylene Blue Agar, abbreviated EMB, was developed by Holt-Harris and Teague. This formula contains lactose and sucrose with two indicator dyes, Eosin Y and Methylene Blue. Levine modified the formula by removing sucrose and doubling the concentration of lactose. Eosin Methylene Blue Agar, Levine is used for testing food and dairy products. This medium is primarily used for the detection and confirmation of coliforms.

 

Formula Liter
Enzymatic Digest of Gelatin 10 g
Lactose 10 g
Dipotassium Phosphate 2 g
Eosin Y 0.4 g
Methylene Blue 0.065 g
Agar 15 g

Final pH: 7.1 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Suspend 37.5 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7103A Eosin Methylene Blue Agar, Levine, 500 g

Reference Material

7103 - Eosin Methylene Blue Agar, Levine, Product Information Sheet [English] [Portuguese]
7103 - Eosin Methylene Blue Agar, Levine, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]

For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.

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